1th Human Performance Wing, Bioeffects Division, Air Force Research Laboratory, Fort Sam Houston, Texas, United States of America.
PLoS One. 2014 Jan 3;9(1):e84614. doi: 10.1371/journal.pone.0084614. eCollection 2014.
The study of living cells may require advanced imaging techniques to track weak and rapidly changing signals. Fundamental to this need is the recent advancement in camera technology. Two camera types, specifically sCMOS and EM-CCD, promise both high signal-to-noise and high speed (>100 fps), leaving researchers with a critical decision when determining the best technology for their application. In this article, we compare two cameras using a live-cell imaging test case in which small changes in cellular fluorescence must be rapidly detected with high spatial resolution. The EM-CCD maintained an advantage of being able to acquire discernible images with a lower number of photons due to its EM-enhancement. However, if high-resolution images at speeds approaching or exceeding 1000 fps are desired, the flexibility of the full-frame imaging capabilities of sCMOS is superior.
研究活细胞可能需要先进的成像技术来跟踪微弱和快速变化的信号。这种需求的基础是最近相机技术的进步。两种相机类型，特别是 sCMOS 和 EM-CCD，都承诺具有高信噪比和高速（>100 fps），这使得研究人员在确定最适合其应用的技术时面临着关键决策。在本文中，我们使用活细胞成像测试案例比较了两种相机，在该案例中，必须以高空间分辨率快速检测细胞荧光的微小变化。由于 EM 增强，EM-CCD 能够用更少的光子获取可分辨的图像，因此保持了优势。但是，如果需要以接近或超过 1000 fps 的速度获得高分辨率图像，则 sCMOS 的全帧成像功能的灵活性更具优势。
