Cabrera C V, Alonso M C, Johnston P, Phillips R G, Lawrence P A
Cell. 1987 Aug 14;50(4):659-63. doi: 10.1016/0092-8674(87)90039-0.
Earlier work suggested that the wingless gene of Drosophila is required for cooperation within discrete groups of cells during development. We show that antisense RNA made from a 3 kb transcript produces wingless mutant phenocopies when injected into wild-type eggs, proving that this transcript executes the wingless function. In the accompanying paper, Rijsewijk et al. report cloning of a Drosophila homolog (Dint-1) of the mouse int-1 gene and show that this gene is identical to wingless+. The partial sequence of the wingless cDNA that we have isolated is identical to part of the complete Dint-1 sequence described by Rijsewijk et al.
早期研究表明，果蝇的无翅基因在发育过程中对于离散细胞群内的协作是必需的。我们发现，由一个3kb转录本制备的反义RNA注射到野生型卵中时会产生无翅突变体表型，这证明该转录本执行无翅基因的功能。在随附论文中，里斯维克等人报道了小鼠int-1基因的果蝇同源基因（Dint-1）的克隆，并表明该基因与无翅基因+相同。我们分离得到的无翅cDNA的部分序列与里斯维克等人描述的完整Dint-1序列的一部分相同。
