Rosenberg U B, Preiss A, Seifert E, Jäckle H, Knipple D C
Nature. 1985;313(6004):703-6. doi: 10.1038/313703a0.
The demonstration that a specific messenger RNA can be functionally inactivated in vivo by hybridization to complementary polynucleotide sequences suggests a direct approach to the study of gene function in cells of higher organisms. The experiments described here were designed to inhibit, by complementary RNA sequences, a specific gene function affecting the fate of the Drosophila embryo. We used the SP6 vector in vitro transcription system to transcribe parts of the normally untranscribed (nonsense) strand of the Krüppel (Kr) gene into complementary Kr RNA (Kr antisense RNA). Wild-type Drosophila embryos, injected with this RNA, developed into phenocopies of Kr mutant embryos.
特定信使核糖核酸（mRNA）可通过与互补多核苷酸序列杂交在体内被功能性灭活，这一发现为研究高等生物细胞中的基因功能提供了一种直接方法。本文所述实验旨在通过互补RNA序列抑制影响果蝇胚胎发育命运的特定基因功能。我们利用SP6载体体外转录系统，将克虏伯氏（Kr）基因正常情况下不转录（无义）链的部分转录成互补的Kr RNA（Kr反义RNA）。注射了这种RNA的野生型果蝇胚胎发育成了Kr突变体胚胎的表型模拟物。
