Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM), Kepala Batas, Pulau Pinang, Malaysia.
Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Southwest Eye Hospital, Southwest Hospital, The Third Military Medical University, Chongqing, P.R. China.
PLoS One. 2014 Jan 7;9(1):e83818. doi: 10.1371/journal.pone.0083818. eCollection 2014.
Eurycoma longifolia Jack has been widely used in traditional medicine for its antimalarial, aphrodisiac, anti-diabetic, antimicrobial and anti-pyretic activities. Its anticancer activity has also been recently reported on different solid tumors, however no anti-leukemic activity of this plant has been reported. Thus the present study assesses the in vitro and in vivo anti-proliferative and apoptotic potentials of E. longifolia on K-562 leukemic cell line. The K-562 cells (purchased from ATCC) were isolated from patients with chronic myelocytic leukemia (CML) were treated with the various fractions (TAF273, F3 and F4) of E. longifolia root methanolic extract at various concentrations and time intervals and the anti-proliferative activity assessed by MTS assay. Flow cytometry was used to assess the apoptosis and cell cycle arrest. Nude mice injected subcutaneously with 10(7) K-562 cells were used to study the anti-leukemic activity of TAF273 in vivo. TAF273, F3 and F4 showed various degrees of growth inhibition with IC50 values of 19, 55 and 62 µg/ml, respectively. TAF273 induced apoptosis in a dose and time dependent manner. TAF273 arrested cell cycle at G1 and S phases. Intraperitoneal administration of TAF273 (50 mg/kg) resulted in a significant growth inhibition of subcutaneous tumor in TAF273-treated mice compared with the control mice (P = 0.024). TAF273 shows potent anti-proliferative activity in vitro and in vivo models of CML and therefore, justifies further efforts to define more clearly the potential benefits of using TAF273 as a novel therapeutic strategy for CML management.
马来西亚国宝东革阿里对治疗疟疾、壮阳、降血糖、抗菌和退热等传统医学有广泛的应用。最近有报道称，它对不同实体瘤具有抗癌活性，但尚未有关于该植物具有抗白血病活性的报道。因此，本研究评估了东革阿里根甲醇提取物的不同部分（TAF273、F3 和 F4）在体外和体内对 K-562 白血病细胞系的增殖抑制和凋亡作用。从慢性髓细胞白血病（CML）患者中分离出 K-562 细胞（购自 ATCC），用不同浓度和时间间隔的东革阿里根甲醇提取物的不同部分（TAF273、F3 和 F4）处理，并通过 MTS 测定法评估抗增殖活性。使用流式细胞术评估细胞凋亡和细胞周期阻滞。用 10(7)个 K-562 细胞皮下注射裸鼠，研究 TAF273 在体内的抗白血病活性。TAF273、F3 和 F4 显示出不同程度的生长抑制，IC50 值分别为 19、55 和 62μg/ml。TAF273 以剂量和时间依赖的方式诱导细胞凋亡。TAF273 将细胞周期阻滞在 G1 和 S 期。与对照组相比，腹腔内给予 TAF273（50mg/kg）可显著抑制 TAF273 治疗组裸鼠皮下肿瘤的生长（P=0.024）。TAF273 在体外和体内 CML 模型中均表现出较强的增殖抑制活性，因此，进一步努力明确将 TAF273 作为 CML 管理的新型治疗策略的潜在益处是合理的。
