一种兼容活检针的变焦距多光子刚性探头，用于深度分辨光学活检。
A biopsy-needle compatible varifocal multiphoton rigid probe for depth-resolved optical biopsy.
机构信息
Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland.
出版信息
J Biophotonics. 2019 Jan;12(1):e201800229. doi: 10.1002/jbio.201800229. Epub 2018 Sep 5.
Abstract
In this work, we report a biopsy-needle compatible rigid probe, capable of performing three-dimensional (3D) two-photon optical biopsy. The probe has a small outer diameter of 1.75 mm and fits inside a gauge-14 biopsy needle to reach internal organs. A carefully designed focus scanning mechanism has been implemented in the rigid probe, which, along with a rapid two-dimensional MEMS scanner, enables 3D imaging. Fast image acquisition up to 10 frames per second is possible, dramatically reducing motion artifacts during in vivo imaging. Equipped with a high-numerical aperture micro-objective, the miniature rigid probe offers a high two-photon resolution (0.833 × 6.11 μm, lateral × axial), a lateral field of view of 120 μm, and an axial focus tuning range of 200 μm. In addition to imaging of mouse internal organs and subcutaneous tumor in vivo, first-of-its-kind depth-resolved two-photon optical biopsy of an internal organ has been successfully demonstrated on mouse kidney in vivo and in situ.
摘要
在这项工作中，我们报告了一种与活检针兼容的刚性探头，能够进行三维（3D）双光子光学活检。该探头外径小至 1.75mm，可以装入 14 号活检针内到达内部器官。在刚性探头中实现了精心设计的聚焦扫描机制，与快速二维 MEMS 扫描仪相结合，实现了 3D 成像。可以实现高达每秒 10 帧的快速图像采集，大大减少了体内成像中的运动伪影。该微型刚性探头配备高数值孔径微物镜，具有高双光子分辨率（0.833×6.11μm，横向×轴向）、120μm 的横向视野和 200μm 的轴向聚焦调节范围。除了对活体小鼠内部器官和皮下肿瘤进行成像外，我们还成功地在活体和原位小鼠肾脏上进行了首例深度分辨双光子光学活检。
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