Chen Li, Hu Jingxiao, Ran Jiabing, Shen Xinyu, Tong Hua
Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Int J Biol Macromol. 2014 Apr;65:1-7. doi: 10.1016/j.ijbiomac.2014.01.003. Epub 2014 Jan 9.
A new in situ precipitation technique was developed to synthesize collagen-silk fibroin/hydroxyapatite nanocomposites. The componential properties and morphological of nanocomposites were investigated. It was revealed that the inorganic phase in the nanocomposite was carbonate-substituted hydroxyapatite with low crystallinity. Morphology studies showed that hydroxyapatite particles with size ranging from 30 to 100 nm were distributed uniformly in the polymer matrix. According to the TEM micrographs, inorganic particles were composed of more fine sub-particles whose diameters were between 2 and 5 nm in size without regular crystallographic orientation. The mechanical properties of the composites were evaluated by measuring their elastic modulus. The data indicated that the elastic modulus of nanocomposites was improved by the addition of silk fibroin. Finally, the cell biocompatibility of the composites was tested in vitro, which showed that they have good biocompatibility. These results suggest that the collagen-silk fibroin/hydroxyapatite nanocomposites are promising biomaterials for bone tissue engineering.
开发了一种新的原位沉淀技术来合成胶原蛋白 - 丝素蛋白/羟基磷灰石纳米复合材料。研究了纳米复合材料的成分特性和形态。结果表明，纳米复合材料中的无机相是低结晶度的碳酸取代羟基磷灰石。形态学研究表明，尺寸范围为30至100nm的羟基磷灰石颗粒均匀分布在聚合物基质中。根据透射电子显微镜照片，无机颗粒由直径在2至5nm之间的更细小的亚颗粒组成，没有规则的晶体取向。通过测量其弹性模量来评估复合材料的力学性能。数据表明，添加丝素蛋白可提高纳米复合材料的弹性模量。最后，在体外测试了复合材料的细胞生物相容性，结果表明它们具有良好的生物相容性。这些结果表明，胶原蛋白 - 丝素蛋白/羟基磷灰石纳米复合材料是骨组织工程中有前途的生物材料。
