Sperandio Guilherme Bento, Melo Reynaldo Magalhães, Gomes Taísa Godoy, Miller Robert Neil Gerard, do Vale Luis Henrique Ferreira, Sousa Marcelo Valle de, Ricart Carlos André Ornelas, Filho Edivaldo Ximenes Ferreira
Laboratory of Enzymology, Department of Cell Biology, University of Brasília, Brasília 70910-900, DF, Brazil.
Laboratory of Protein Chemistry and Biochemistry, Department of Cellular Biology, University of Brasília, Brasilia 70910-900, DF, Brazil.
J Fungi (Basel). 2024 Sep 28;10(10):677. doi: 10.3390/jof10100677.
The spectrum of enzymes required for complete lignocellulosic waste hydrolysis is too diverse to be secreted by a single organism. An alternative is to employ fungal co-cultures to obtain more diverse and complete enzymatic cocktails without the need to mix enzymes during downstream processing. This study evaluated the co-cultivation of and RUT-C30 in different conditions using sugarcane bagasse as the carbon source. The resulting enzymatic cocktails were characterized according to the impact of strain inoculation time on enzymatic activities and proteome composition. Data revealed that the profile of each enzymatic extract was highly dependent on the order in which the participating fungi were inoculated. Some of the co-cultures exhibited higher enzyme activities compared to their respective monocultures for enzymes such as CMCase, pectinase, β-glucosidase, and β-xylosidase. Analysis of the RUT-C30 and co-culture secretome resulted in the identification of 167 proteins, with 78 from and 89 from . In agreement with the enzymatic results, proteome analysis also revealed that the timing of inoculation greatly influences the overall secretome, with a predominance of RUT-C30 proteins when first inoculated or in simultaneous inoculation.
完全水解木质纤维素废料所需的酶谱过于多样，无法由单一生物体分泌。一种替代方法是采用真菌共培养，以获得更多样化和完整的酶混合物，而无需在下游加工过程中混合酶。本研究以甘蔗渣为碳源，评估了里氏木霉和RUT-C30在不同条件下的共培养。根据菌株接种时间对酶活性和蛋白质组组成的影响，对所得的酶混合物进行了表征。数据显示，每种酶提取物的概况高度依赖于参与共培养的真菌的接种顺序。对于羧甲基纤维素酶、果胶酶、β-葡萄糖苷酶和β-木糖苷酶等酶，一些共培养物与其各自的单培养物相比表现出更高的酶活性。对里氏木霉和RUT-C30共培养物的分泌蛋白质组进行分析，鉴定出167种蛋白质，其中78种来自里氏木霉，89种来自RUT-C30。与酶学结果一致，蛋白质组分析还显示，接种时间对整体分泌蛋白质组有很大影响，首次接种或同时接种时，RUT-C30蛋白质占主导地位。
