Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Virology. 2014 Jan 20;449:120-32. doi: 10.1016/j.virol.2013.11.017. Epub 2013 Nov 28.
Classical inbred mice are extensively used for virus research. However, we recently found that some wild-derived inbred mouse strains are more susceptible than classical strains to monkeypox virus. Experiments described here indicated that the 50% lethal dose of vaccinia virus (VACV) and cowpox virus (CPXV) were two logs lower in wild-derived inbred CAST/Ei mice than classical inbred BALB/c mice, whereas there was little difference in the susceptibility of the mouse strains to herpes simplex virus. Live bioluminescence imaging was used to follow spread of pathogenic and attenuated VACV strains and CPXV virus from nasal passages to organs in the chest and abdomen of CAST/Ei mice. Luminescence increased first in the head and then simultaneously in the chest and abdomen in a dose-dependent manner. The spreading kinetics was more rapid with VACV than CPXV although the peak photon flux was similar. These data suggest advantages of CAST/Ei mice for orthopoxvirus studies.
经典近交系小鼠被广泛用于病毒研究。然而，我们最近发现，一些野生来源的近交系小鼠比经典近交系 BALB/c 小鼠更容易感染猴痘病毒。本文描述的实验表明，在野生来源的近交系 CAST/Ei 小鼠中，天花病毒（VACV）和牛痘病毒（CPXV）的半数致死剂量（LD50）比经典近交系 BALB/c 小鼠低 2 个对数，而这两种小鼠对单纯疱疹病毒的敏感性差异很小。活生物发光成像用于追踪致病性和减毒 VACV 株和 CPXV 病毒从鼻腔传播到胸部和腹部器官。发光首先在头部增加，然后以剂量依赖性的方式同时在胸部和腹部增加。VACV 的传播动力学比 CPXV 更快，尽管峰值光子通量相似。这些数据表明 CAST/Ei 小鼠在正痘病毒研究中的优势。
