伤寒沙门氏菌和肠炎沙门氏菌的毒力分型比较。
Comparative Virulotyping of Salmonella typhi and Salmonella enteritidis.
机构信息
Microbiology Division, Faculty of Science, Institute of Biological Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Biomedical Science and Molecular Microbiology Laboratory, Institute of Graduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.
出版信息
Indian J Microbiol. 2013 Dec;53(4):410-7. doi: 10.1007/s12088-013-0407-y. Epub 2013 Apr 23.
Members of Salmonella enterica are important foodborne pathogens of significant public health concern worldwide. This study aimed to determine a range of virulence genes among typhoidal (S. typhi) and non-typhoidal (S. enteritidis) strains isolated from different geographical regions and different years. A total of 87 S. typhi and 94 S. enteritidis strains were tested for presence of 22 virulence genes by employing multiplex PCR and the genetic relatedness of these strains was further characterized by REP-PCR. In S. typhi, invA, prgH, sifA, spiC, sopB, iroN, sitC, misL, pipD, cdtB, and orfL were present in all the strains, while sopE, agfC, agfA, sefC, mgtC, and sefD were present in 98.8, 97.7, 90.8, 87.4, 87.4 and 17.2 %, of the strains, respectively. No lpfA, lpfC, pefA, spvB, or spvC was detected. Meanwhile, in S. enteritidis, 15 genes, agfA, agfC, invA, lpfA, lpfC, sefD, prgH, spiC, sopB, sopE, iroN, sitC, misL, pipD, and orfL were found in all S. enteritidis strains 100 %, followed by sifA and spvC 98.9 %, pefA, spvB and mgtC 97.8 %, and sefC 90.4 %. cdtB was absent from all S. enteritidis strains tested. REP-PCR subtyped S. typhi strains into 18 REP-types and concurred with the virulotyping results in grouping the strains, while in S. enteritidis, REP-PCR subtyped the strains into eight profiles and they were poorly distinguishable between human and animal origins. The study showed that S. typhi and S. enteritidis contain a range of virulence factors associated with pathogenesis. Virulotyping is a rapid screening method to identify and profile virulence genes in Salmonella strains, and improve an understanding of potential risk for human and animal infections.
肠沙门氏菌成员是全球重要的食源性病原体，对公共健康构成重大威胁。本研究旨在确定来自不同地理区域和不同年份的伤寒（S. typhi）和非伤寒（S. enteritidis）菌株中的一系列毒力基因。采用多重 PCR 检测了 87 株 S. typhi 和 94 株 S. enteritidis 菌株中 22 种毒力基因的存在情况，并进一步采用重复回文序列 PCR（REP-PCR）对这些菌株的遗传相关性进行了特征描述。在 S. typhi 中，invA、prgH、sifA、spiC、sopB、iroN、sitC、misL、pipD、cdtB 和 orfL 存在于所有菌株中，而 sopE、agfC、agfA、sefC、mgtC 和 sefD 存在于 98.8%、97.7%、90.8%、87.4%、87.4%和 17.2%的菌株中。未检测到 lpfA、lpfC、pefA、spvB 或 spvC。同时，在 S. enteritidis 中，15 种基因，即 agfA、agfC、invA、lpfA、lpfC、sefD、prgH、spiC、sopB、sopE、iroN、sitC、misL、pipD 和 orfL 存在于所有 S. enteritidis 菌株中，100%，其次是 sifA 和 spvC，98.9%，pefA、spvB 和 mgtC，97.8%，sefC，90.4%。cdtB 不存在于所有测试的 S. enteritidis 菌株中。REP-PCR 将 S. typhi 菌株分为 18 种 REP 型，并与毒力分型结果一致，将菌株分组，而在 S. enteritidis 中，REP-PCR 将菌株分为 8 种谱型，它们在人类和动物来源之间很难区分。该研究表明，S. typhi 和 S. enteritidis 含有一系列与发病机制相关的毒力因子。毒力分型是一种快速筛选方法，可用于鉴定和分析沙门氏菌菌株中的毒力基因，并提高对人类和动物感染潜在风险的认识。
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