Silva M A R, Silva E C B, Gurgel A P A D, Nascimento K C G, Freitas A C
Laboratório de Estudos Moleculares e Terapia Experimental, Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE Brazil.
Laboratório de Andrologia, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, PE Brazil.
Virusdisease. 2014 Jan;25(1):125-8. doi: 10.1007/s13337-013-0185-5. Epub 2013 Nov 30.
Papillomaviruses are found in epithelial lesions and are linked to different carcinogenic processes in humans and other animals. Although BPV has been characterized as epitheliotropic, the presence of viral DNA has been detected in other tissues and fluids, such as fresh semen. The aim of this study was to evaluate the presence and expression of BPV in sperm cells of bulls (Bos taurus) asymptomatic for papillomatosis. A PCR assay was carried out with specific primers to test BPV2 in 26 semen samples. The presence of BPV transcripts was assessed by RT-PCR to E2 and E5 genes. BPV2 DNA was detected in nine out of 26 samples and the expression of E2 and E5 were detected in five out of nine BPV positive samples. This is the first record of BPV2 expression in bull sperm cells.
乳头瘤病毒存在于上皮病变中，并与人类和其他动物的不同致癌过程有关。尽管牛乳头瘤病毒（BPV）已被鉴定为嗜上皮性，但在其他组织和液体（如新鲜精液）中也检测到了病毒DNA。本研究的目的是评估无症状乳头瘤病的公牛（Bos taurus）精子细胞中BPV的存在和表达。用特异性引物进行PCR检测，以检测26份精液样本中的BPV2。通过逆转录PCR评估E2和E5基因的BPV转录本的存在情况。在26份样本中的9份中检测到BPV2 DNA，在9份BPV阳性样本中的5份中检测到E2和E5的表达。这是BPV2在公牛精子细胞中表达的首次记录。
