Department of Genetics, Center for Biological Sciences, Federal University of Pernambuco, Recife, PE, Brazil.
Transbound Emerg Dis. 2014 Aug;61(4):329-33. doi: 10.1111/tbed.12036. Epub 2012 Dec 4.
Papillomavirus (PV) are double-stranded DNA viruses that can cause both benignant and malignant tumours in mammals. Twelve genotypes of bovine papillomavirus (BPV1-12) have been identified so far. The presence of BPV1 and 2 has been found in the body fluids of cattle and horses. The aim of this study is to investigate the presence of BPV DNA and the expression of viral genes in the blood and sperm cells of healthy horses using PCR and RT-PCR. BPV-1 or 2 was detected in 14 of 70 blood samples (20%) and in 11 of 31 semen samples (35%). In five of fourteen blood samples, the E5 expression tested positive, while no blood sample was positive for L1 expression. Four of 11 (36%) semen cell samples proved to be positive for E5 expression, while no gene expression in L1 could be detected. This is the first study that shows BPV1 gene expression in the blood and semen of healthy horses. Our data illustrate the need for a better understanding of the presence of BPV in non-epithelial tissues of horses and their role in the vertical and horizontal transmission of these viruses.
乳头瘤病毒（PV）是双链 DNA 病毒，可导致哺乳动物的良性和恶性肿瘤。迄今为止，已鉴定出 12 种牛乳头瘤病毒（BPV1-12）基因型。已在牛和马的体液中发现了 BPV1 和 2 的存在。本研究旨在使用 PCR 和 RT-PCR 检测健康马血液和精子细胞中 BPV DNA 的存在和病毒基因的表达。在 70 份血液样本中的 14 份（20%）和 31 份精液样本中的 11 份（35%）中检测到 BPV-1 或 2。在 14 份血液样本中的 5 份中，E5 表达呈阳性，而 L1 表达没有阳性样本。在 11 份精液细胞样本中的 4 份中，E5 表达呈阳性，而 L1 中未检测到基因表达。这是第一项表明 BPV1 基因在健康马的血液和精液中表达的研究。我们的数据表明，需要更好地了解 BPV 在马的非上皮组织中的存在及其在这些病毒的垂直和水平传播中的作用。
