Yamazaki H, Takagi S, Oh N, Hoshino Y, Hosoya K, Okumura M
Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sappro, Hokkaido, Japan.
J Vet Intern Med. 2014 Jan-Feb;28(1):204-10. doi: 10.1111/jvim.12244. Epub 2013 Nov 13.
Definitive diagnosis of histiocytic sarcoma (HS) in dogs is relatively difficult by conventional histopathological examination because objective features of HS are not well defined.
Quantitative analysis of mRNA expression of selected cellular surface antigens (SAs) specific to HS in dogs can facilitate objective and rapid diagnosis.
Dogs with HS (n = 30) and dogs without HS (n = 36), including those with other forms of lymphoma (n = 4), inflammatory diseases (n = 6), and other malignant neoplasias (n = 26).
Retrospective clinical observational study. Specimens were collected by excisional biopsy, needle core biopsy, or fine needle aspiration. To determine HS detection efficacy, mRNA expression levels of selected SAs specific to HS in dogs, including MHC class IIα, CD11b, CD11c, and CD86, were quantitatively analyzed using real-time quantitative polymerase chain reaction.
Each SA mRNA expression level was significantly higher in HS dogs than in non-HS dogs (P = .0082). Cutoff values for discriminating between HS and non-HS dogs based on these expression levels were calculated on the basis of receiver-operating characteristic analysis. Accuracy of the cutoff values, including MHC class IIα, CD11b, CD11c, and CD86, was 87.9, 86.4, 86.4, and 84.8%, respectively.
Our results suggest that quantitative analysis of mRNA expression of the selected SAs could be an adjunctive diagnostic technique with high diagnostic accuracy for HS in dogs. Substantial investigation is required for exclusion of diseases with similar cell types of origin to lymphoma.
由于组织细胞肉瘤（HS）的客观特征尚未明确界定，通过传统组织病理学检查对犬类组织细胞肉瘤进行确诊相对困难。
对犬类HS特异性选择的细胞表面抗原（SAs）的mRNA表达进行定量分析，有助于客观、快速地诊断。
患有HS的犬（n = 30）和未患HS的犬（n = 36），包括患有其他形式淋巴瘤的犬（n = 4）、炎症性疾病的犬（n = 6）以及其他恶性肿瘤的犬（n = 26）。
回顾性临床观察研究。通过切除活检、针芯活检或细针穿刺采集样本。为确定HS检测效能，使用实时定量聚合酶链反应对犬类HS特异性选择的SAs（包括MHC IIα类、CD11b、CD11c和CD86）的mRNA表达水平进行定量分析。
HS犬的每种SA mRNA表达水平均显著高于非HS犬（P = .0082）。基于受试者工作特征分析，计算出基于这些表达水平区分HS犬和非HS犬的临界值。包括MHC IIα类、CD11b、CD11c和CD86在内的临界值的准确率分别为87.9%、86.4%、86.4%和84.8%。
我们的结果表明，对所选SAs的mRNA表达进行定量分析可能是一种对犬类HS具有高诊断准确性的辅助诊断技术。需要进行大量研究以排除起源细胞类型与淋巴瘤相似的疾病。
