印度菖蒲种质的分子和化学特征分析。

Molecular and chemical profiling of 'sweet flag' (Acorus calamus L.) germplasm from India.

机构信息

Molecular Systematics Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, India 226001.

Pharmacognosy and Ethnopharmacology Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, India 226001.

出版信息

Physiol Mol Biol Plants. 2013 Apr;19(2):231-7. doi: 10.1007/s12298-013-0164-8.

DOI:10.1007/s12298-013-0164-8

PMID:24431490

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3656192/

Abstract

In the present study, molecular (DAMD and ISSR) and chemical (α and β-asarone contents) markers were used to characterize the A. calamus genotypes procured from different parts of India. The cumulative analysis carried out for both DAMD and ISSR markers revealed 24.71 % polymorphism across all genotypes of A. calamus. The clustering patterns of the genotypes in the UPGMA tree showed that the genotypes are diverse, and did not show any specific correlation with their geographical provenances, reflecting the low level of genetic diversity and a high genetic differentiation among the genotypes from the same localities. All the 27 genotypes of A. calamus were also analyzed for α and β-asarone contents, and percentage of essential oil. The genotype (Ac13) from Kullu (Himachal Pradesh) showed maximum (9.5 %) percentage of oil, whereas corresponding minimum (2.8 %) was obtained from the genotypes from Pangthang (Sikkim). Similarly, the highest α and β-asarone contents (16.82 % and 92.12 %) were obtained from genotypes from Renuka (Himachal Pradesh) and Udhampur (Jammu & Kashmir), while lowest α and β-asarone contents (0.83 % and 65.96 %) resulted from Auranwa (Uttar Pradesh) and Pangthang (Sikkim) genotypes, respectively. A. calamus harbours tremendous economic value, and it is therefore, important to identify the genotypes with low α and β-asarone contents for its commercial utilization. Further, this study will help in evaluation and documentation of a large number of diverse genotypes for their value traits.

摘要

在本研究中，使用分子（DAMD 和 ISSR）和化学（α-和β-细辛脑含量）标记物来表征从印度不同地区获得的菖蒲基因型。对 DAMD 和 ISSR 标记物进行的累积分析显示，所有菖蒲基因型的多态性为 24.71%。UPGMA 树中基因型的聚类模式表明，基因型是多样的，与它们的地理起源没有任何特定的相关性，反映了来自同一地点的基因型遗传多样性低，遗传分化高。还分析了所有 27 个菖蒲基因型的α-和β-细辛脑含量以及精油百分比。来自库鲁（喜马偕尔邦）的基因型（Ac13）表现出最高的油（9.5%），而来自蓬塘（锡金）的基因型则表现出最低的油（2.8%）。同样，来自 Renuka（喜马偕尔邦）和 Udhampur（查谟和克什米尔）的基因型的α-和β-细辛脑含量最高（分别为 16.82%和 92.12%），而来自 Auranwa（北方邦）和蓬塘（锡金）的基因型的α-和β-细辛脑含量最低（分别为 0.83%和 65.96%）。菖蒲具有巨大的经济价值，因此，识别具有低α-和β-细辛脑含量的基因型对于其商业利用非常重要。此外，这项研究将有助于评估和记录大量不同基因型的价值特征。