Mehrotra S, Mishra K P, Maurya R, Srimal R C, Yadav V S, Pandey R, Singh V K
Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow 226 014, India.
Int Immunopharmacol. 2003 Jan;3(1):53-61. doi: 10.1016/s1567-5769(02)00212-6.
Modulation of immune response to alleviate disease has been of interest since long. Plant extracts have been widely investigated for possible immunomodulatory properties. We have evaluated the anticellular and immunomodulatory properties of ethanolic extract of Acorus calamus rhizome. This extract inhibited proliferation of mitogen (phytohaemagglutinin; PHA) and antigen (purified protein derivative; PPD)-stimulated human peripheral blood mononuclear cells (PBMCs). In addition, A. calamus extract inhibited growth of several cell lines of mouse and human origin. It also inhibited production of nitric oxide (NO), interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha). Intracytoplasmic interferon-gamma (IFN-gamma) and expression of cell surface markers, CD16 and HLA-DR, on human PBMC, were not affected on treatment with A. calamus extract but CD25 expression was down regulated. Our study demonstrates the antiproliferative and immunosuppressive potential of ethanolic extract of A. calamus rhizome in vitro.
长期以来，调节免疫反应以减轻疾病一直备受关注。植物提取物因其可能具有的免疫调节特性而受到广泛研究。我们评估了菖蒲根茎乙醇提取物的抗细胞和免疫调节特性。该提取物抑制有丝分裂原（植物血凝素；PHA）和抗原（纯化蛋白衍生物；PPD）刺激的人外周血单个核细胞（PBMC）的增殖。此外，菖蒲提取物抑制多种小鼠和人源细胞系的生长。它还抑制一氧化氮（NO）、白细胞介素-2（IL-2）和肿瘤坏死因子-α（TNF-α）的产生。菖蒲提取物处理后，人PBMC胞浆内的干扰素-γ（IFN-γ）以及细胞表面标志物CD16和HLA-DR的表达未受影响，但CD25表达下调。我们的研究证明了菖蒲根茎乙醇提取物在体外具有抗增殖和免疫抑制潜力。
