不稳定溶源菌 Serratia 噬菌体 η 的基因组和蛋白质组。
The genome and proteome of Serratia bacteriophage η which forms unstable lysogens.
机构信息
Department of Molecular & Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.
出版信息
Virol J. 2014 Jan 16;11:6. doi: 10.1186/1743-422X-11-6.
BACKGROUND
Serratia marcescens phage η is a temperate unclassified member of the Siphoviridae which had been reported as containing hypermodified guanine residues.
METHODS
The DNA was characterized by enzymatic digestion followed by HPLC analysis of the nucleoside composition, and by DNA sequencing and proteomic analysis. Its ability to form stable lysogens and integrate was also investigated.
RESULTS
Enzymatic digestion and HPLC analysis revealed phage η DNA did not contain modified bases. The genome sequence of this virus, determined using pyrosequencing, is 42,724 nucleotides in length with a mol% GC of 49.9 and is circularly permuted. Sixty-nine putative CDSs were identified of which 19 encode novel proteins. While seven close genetic relatives were identified, they shared sequence similarity with only genes 40 to 69 of the phage η genome, while gp1 to gp39 shared no conserved relationship. The structural proteome, determined by SDS-PAGE and mass spectrometry, revealed seven unique proteins. This phage forms very unstable lysogens with its host S. marcescens.
背景
粘质沙雷氏菌噬菌体 η 是一种未分类的温和噬菌体，属于长尾噬菌体科，曾被报道含有超修饰的鸟嘌呤残基。
方法
通过酶切和 HPLC 分析核苷组成，以及 DNA 测序和蛋白质组学分析来对 DNA 进行特征分析。还研究了其形成稳定溶原菌和整合的能力。
结果
酶切和 HPLC 分析表明噬菌体 η DNA 不含修饰碱基。该病毒的基因组序列使用焦磷酸测序确定，长度为 42724 个核苷酸，GC 含量为 49.9%，呈环状排列。鉴定出 69 个可能的 CDS，其中 19 个编码新蛋白。虽然鉴定出了 7 个密切相关的遗传亲属，但它们与噬菌体 η 基因组的基因 40 到 69 仅具有序列相似性，而 gp1 到 gp39 没有保守关系。通过 SDS-PAGE 和质谱法确定的结构蛋白质组显示有 7 种独特的蛋白质。这种噬菌体与它的宿主粘质沙雷氏菌形成非常不稳定的溶原菌。
Zotero 插件