Athanasou N A, Quinn J, Heryet A, Woods C G, McGee J O
University of Oxford, Nuffield Department of Pathology, John Radcliffe Hospital.
J Clin Pathol. 1987 Aug;40(8):874-8. doi: 10.1136/jcp.40.8.874.
The effects of several strong acids, weak acids, a proprietary decalcifier, and edetic acid on the immunohistochemical staining of cryostat and fixed paraffin embedded sections of tissue from a variety of normal and pathological calcified and uncalcified specimens were studied. Even decalcification in strong acids (HCl, HN03, 5% trichloracetic acid, HCl-edetic acid did not diminish the reactivity of many useful antigens (including leucocyte common antigen, intermediate filaments, S100 protein and epithelial membrane antigen). Weaker acids (formic acid, acetic acid) and edetic acid decalcified more slowly and generally showed greater preservation of antigenic reactivity with better morphology and staining quality. Trichloracetic acid was also useful as a quick one step fixation and decalcifying agent for both cryostat and routinely processed sections. Knowledge of the preservation of antigenic reactivity in decalcified tissue will be useful in the diagnosis of tumours of uncertain histogenesis and origin which affect calcified tissues.
研究了几种强酸、弱酸、一种专利脱钙剂以及乙二胺四乙酸（EDTA）对来自各种正常和病理钙化及未钙化标本的组织冷冻切片和固定石蜡包埋切片免疫组织化学染色的影响。即使在强酸（盐酸、硝酸、5%三氯乙酸、盐酸 - 乙二胺四乙酸）中脱钙，许多有用抗原（包括白细胞共同抗原、中间丝、S100蛋白和上皮膜抗原）的反应性也不会降低。较弱的酸（甲酸、乙酸）和乙二胺四乙酸脱钙较慢，通常显示出对抗原反应性的更好保存，形态和染色质量更佳。三氯乙酸对于冷冻切片和常规处理切片也是一种有用的快速一步固定和脱钙剂。了解脱钙组织中抗原反应性的保存情况对于诊断影响钙化组织的组织发生和起源不确定的肿瘤将是有用的。
