Monestier M, Bonin B, Migliorini P, Dang H, Datta S, Kuppers R, Rose N, Maurer P, Talal N, Bona C
Department of Microbiology, Mount Sinai School of Medicine, New York 10029.
J Exp Med. 1987 Oct 1;166(4):1109-24. doi: 10.1084/jem.166.4.1109.
We examined the binding to foreign antigens and the expression of crossreactive idiotypes by a panel of 20 murine monoclonal autoantibodies encoded by V genes from the VH J558 family. 9 of 20 antibodies bound to foreign antigens such as bacterial polysaccharides, poly(Glu50, Tyr50), poly(Glu54,Lys37,Phe9), arsonate, and lysozyme, known to interact with antibodies encoded by genes from the VH J558 family. A high proportion of our panel of autoantibodies expressed crossreactive idiotypes originally borne by monoclonal rheumatoid factors, anti-Sm, and anti-DNA antibodies, all encoded by V genes from the VH J558 family. Some of these VH J558+ autoantibodies shared crossreactive idiotypes with VH J558+ antibodies directed against foreign antigens such as influenza virus hemagglutinin, poly(Glu60,Ala30,Tyr10), arsonate, and dextran. The implications of these findings are discussed with respect to the process of activation of self-reactive clones.
我们检测了一组由VH J558家族V基因编码的20种鼠单克隆自身抗体与外来抗原的结合情况以及交叉反应性独特型的表达。20种抗体中有9种与外来抗原结合，如细菌多糖、聚（Glu50，Tyr50）、聚（Glu54，Lys37，Phe9）、砷酸盐和溶菌酶，已知这些抗原与VH J558家族基因编码的抗体相互作用。我们的自身抗体组中有很大比例表达了最初由单克隆类风湿因子、抗Sm和抗DNA抗体所携带的交叉反应性独特型，这些抗体均由VH J558家族的V基因编码。其中一些VH J558 +自身抗体与针对外来抗原（如流感病毒血凝素、聚（Glu60，Ala30，Tyr10）、砷酸盐和葡聚糖）的VH J558 +抗体共享交叉反应性独特型。我们结合自身反应性克隆的激活过程对这些发现的意义进行了讨论。
