雪峰乌骨鸡的完整线粒体基因组
The complete mitochondrial genome of the Xuefeng black-boned chicken.
作者信息
Liu Li-Li, Xie Hong-Bing, Yang Yong-Sheng, Yu Qi-Fang, He Jian-Hua
机构信息
a College of Animal Science and Technology, Hunan Agricultural University , Changsha , P.R. China and.
b College of Life Science, Hunan University of Science and Technology , Xiangtan , P.R. China.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):30-1. doi: 10.3109/19401736.2013.869679. Epub 2014 Jan 17.
Xuefeng black-boned chicken is one of the famous native breeds in China. In this work, we reported the complete mitochondrial genome sequence of the Xuefeng black-boned chicken for the first time, which was determined through PCR-based method. The total length of the mitogenome was 16,783 bp, with the base composition of 30.24% for A, 23.72% for T, 32.52% for C, 13.53% for G, in the order C > A > T > G feature occurring in the Xuefeng black-boned chicken. It contained the typical structure, including 2 ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes and 1 non-coding control region (D-loop region). It was similar to the gene arrangement in Silky chicken. The complete mitochondrial genome sequence of the Xuefeng black-boned chicken provided an important data for further study on the genetic mechanism.
雪峰乌鸡是中国著名的地方品种之一。在本研究中，我们首次报道了通过基于PCR的方法测定的雪峰乌鸡线粒体基因组全序列。该线粒体基因组全长16783 bp，碱基组成为：A占30.24%，T占23.72%，C占32.52%，G占13.53%，呈现出雪峰乌鸡中C＞A＞T＞G的特征。它具有典型的结构，包括2个核糖体RNA基因、13个蛋白质编码基因、22个转运RNA基因和1个非编码控制区（D-loop区）。其基因排列与丝羽乌骨鸡相似。雪峰乌鸡线粒体基因组全序列为进一步研究其遗传机制提供了重要数据。
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