博来霉素与DNA的序列选择性结合。
Sequence-selective binding of phleomycin to DNA.
作者信息
Fox K R, Grigg G W, Waring M J
机构信息
Department of Pharmacology, University of Cambridge, U.K.
出版信息
Biochem J. 1987 May 1;243(3):847-51. doi: 10.1042/bj2430847.
Abstract
The binding of phleomycin and bleomycin to DNA has been investigated by studying their effects on cleavage by DNAase I and micrococcal nuclease. In the presence of cobalt, cleavage of DNA by the antibiotics is suppressed, yet they still provide protection from nuclease attack in regions surrounding the drug cleavage sites. We conclude that cleavage by phleomycin occurs at bonds around which the antibiotic is already selectively bound.
摘要
通过研究博来霉素和争光霉素对DNA酶I及微球菌核酸酶切割作用的影响，对它们与DNA的结合情况进行了研究。在钴存在的情况下，抗生素对DNA的切割作用受到抑制，但它们仍能在药物切割位点周围区域提供免受核酸酶攻击的保护作用。我们得出结论，博来霉素的切割发生在抗生素已选择性结合的键周围。
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