• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于检测药物对癌细胞系细胞毒性的iCELLigence实时细胞分析系统。

iCELLigence real-time cell analysis system for examining the cytotoxicity of drugs to cancer cell lines.

作者信息

Türker Şener Leyla, Albeniz Gürcan, Dinç Bircan, Albeniz Işil

机构信息

Department of Biophysics, Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey.

Department of General Surgery, Cerrahpaşa Faculty of Medicine, Istanbul University, 34096 Istanbul, Turkey.

出版信息

Exp Ther Med. 2017 Sep;14(3):1866-1870. doi: 10.3892/etm.2017.4781. Epub 2017 Jul 11.

DOI:10.3892/etm.2017.4781
PMID:28962095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5609197/
Abstract

The recently developed iCELLigence™ real-time cell analyzer (RTCA) can be used for the label-free real-time monitoring of cancer cell proliferation, viability, invasion and cytotoxicity. The RTCA system uses 16-well microtiter plates with a gold microelectrode biosensor array that measures impedance when cells adhere to the microelectrodes causing an alternating current. By measuring the electric field generated in this process, the RTCA system can be used for the analysis of cell proliferation, viability, morphology and migration. The present review aimed to summarize the working method of the RTCA system, in addition to discussing the research performed using the system for various applications, including cancer drug discovery via measuring cytotoxicity.

摘要

最近开发的iCELLigence™实时细胞分析仪（RTCA）可用于对癌细胞增殖、活力、侵袭和细胞毒性进行无标记实时监测。RTCA系统使用带有金微电极生物传感器阵列的16孔微量滴定板，当细胞粘附到微电极上产生交流电时，该阵列会测量阻抗。通过测量此过程中产生的电场，RTCA系统可用于分析细胞增殖、活力、形态和迁移。本综述旨在总结RTCA系统的工作方法，此外还讨论了使用该系统进行各种应用的研究，包括通过测量细胞毒性进行癌症药物发现。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d30d/5609197/883e05aff2ab/etm-14-03-1866-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d30d/5609197/3f3f07d2d2ff/etm-14-03-1866-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d30d/5609197/b63f42047284/etm-14-03-1866-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d30d/5609197/2b1665a7a9d1/etm-14-03-1866-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d30d/5609197/883e05aff2ab/etm-14-03-1866-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d30d/5609197/3f3f07d2d2ff/etm-14-03-1866-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d30d/5609197/b63f42047284/etm-14-03-1866-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d30d/5609197/2b1665a7a9d1/etm-14-03-1866-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d30d/5609197/883e05aff2ab/etm-14-03-1866-g03.jpg

相似文献

1
iCELLigence real-time cell analysis system for examining the cytotoxicity of drugs to cancer cell lines.用于检测药物对癌细胞系细胞毒性的iCELLigence实时细胞分析系统。
Exp Ther Med. 2017 Sep;14(3):1866-1870. doi: 10.3892/etm.2017.4781. Epub 2017 Jul 11.
2
Evaluation of IC levels immediately after treatment with anticancer reagents using a real-time cell monitoring device.使用实时细胞监测设备评估抗癌试剂治疗后即刻的IC水平。
Exp Ther Med. 2019 Oct;18(4):3197-3205. doi: 10.3892/etm.2019.7876. Epub 2019 Aug 13.
3
Evaluation of impedance-based label-free technology as a tool for pharmacology and toxicology investigations.基于阻抗的无标记技术作为药理学和毒理学研究工具的评价。
Biosensors (Basel). 2013 Mar 15;3(1):132-56. doi: 10.3390/bios3010132. eCollection 2013 Mar.
4
Dynamic assessment of cell viability, proliferation and migration using real time cell analyzer system (RTCA).采用实时细胞分析系统（RTCA）对细胞活力、增殖和迁移进行动态评估。
Cytotechnology. 2015 Mar;67(2):379-86. doi: 10.1007/s10616-014-9692-5. Epub 2014 Jan 19.
5
Reuse of E-plate cell sensor arrays in the xCELLigence Real-Time Cell Analyzer.xCELLigence实时细胞分析仪中E-plate细胞传感器阵列的重复使用。
Biotechniques. 2016 Sep 1;61(3):117-22. doi: 10.2144/000114450. eCollection 2016.
6
Comparative analysis of dynamic cell viability, migration and invasion assessments by novel real-time technology and classic endpoint assays.新型实时技术与经典终点分析评估细胞活力、迁移和侵袭的比较分析。
PLoS One. 2012;7(10):e46536. doi: 10.1371/journal.pone.0046536. Epub 2012 Oct 19.
7
Analysis of NK-92 cytotoxicity in nasopharyngeal carcinoma cell lines and patient-derived xenografts using impedance-based growth method.使用基于阻抗的生长方法分析NK-92对鼻咽癌细胞系和患者来源异种移植瘤的细胞毒性。
Heliyon. 2023 Jun 25;9(7):e17480. doi: 10.1016/j.heliyon.2023.e17480. eCollection 2023 Jul.
8
Application of Real-Time Cell Electronic Analysis System in Modern Pharmaceutical Evaluation and Analysis.实时细胞电子分析系统在现代药物评价与分析中的应用。
Molecules. 2018 Dec 11;23(12):3280. doi: 10.3390/molecules23123280.
9
Real-time cell-microelectronic sensing of nanoparticle-induced cytotoxic effects.实时细胞-微电子传感技术检测纳米颗粒诱导的细胞毒性效应。
Anal Chim Acta. 2013 Jul 30;789:83-90. doi: 10.1016/j.aca.2013.06.002. Epub 2013 Jun 18.
10
The xCELLigence system for real-time and label-free monitoring of cell viability.用于实时无标记监测细胞活力的xCELLigence系统。
Methods Mol Biol. 2011;740:33-43. doi: 10.1007/978-1-61779-108-6_6.

引用本文的文献

1
Exploring the Heterogeneity of Cancer-Associated Fibroblasts via Development of Patient-Derived Cell Culture of Breast Cancer.通过乳腺癌患者来源的细胞培养探索癌症相关成纤维细胞的异质性
Int J Mol Sci. 2025 Aug 12;26(16):7789. doi: 10.3390/ijms26167789.
2
Bacterial products initiation of alpha-synuclein pathology: an in vitro study.细菌产物引发α-突触核蛋白病变：一项体外研究
Sci Rep. 2024 Dec 5;14(1):30306. doi: 10.1038/s41598-024-81020-x.
3
PLCγ1 deficiency in chondrocytes accelerates the age-related changes in articular cartilage and subchondral bone.

本文引用的文献

1
Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs.葡萄牙南部五种草药的抗菌、抗氧化、抗增殖特性及锌含量
Pharm Biol. 2017 Dec;55(1):114-123. doi: 10.1080/13880209.2016.1230636. Epub 2016 Sep 22.
2
Cytotoxicity and mitogenicity assays with real-time and label-free monitoring of human granulosa cells with an impedance-based signal processing technology intergrating micro-electronics and cell biology.采用基于阻抗的信号处理技术，结合微电子学和细胞生物学，对人颗粒细胞进行细胞毒性和促有丝分裂活性测定，并进行实时无标记监测。
Reprod Toxicol. 2016 Apr;60:82-91. doi: 10.1016/j.reprotox.2015.12.005. Epub 2015 Dec 30.
3
软骨细胞 PLCγ1 缺失加速关节软骨和软骨下骨的年龄相关性变化。
J Cell Mol Med. 2024 Aug;28(16):e70027. doi: 10.1111/jcmm.70027.
4
Single-Use, Metabolite Absorbing, Resonant Transducer (SMART) Culture Vessels for Label-Free, Continuous Cell Culture Progression Monitoring.一次性使用、代谢物吸收、谐振式换能器（SMART）培养容器，用于无标记、连续的细胞培养过程监测。
Adv Sci (Weinh). 2024 Aug;11(32):e2401260. doi: 10.1002/advs.202401260. Epub 2024 Jun 20.
5
Epithelial-to-mesenchymal transition and NF-kB pathways are promoted by a mutant form of DDB2, unable to bind PCNA, in UV-damaged human cells.在紫外线损伤的人类细胞中，无法结合 PCNA 的 DDB2 突变体形式促进上皮-间充质转化和 NF-κB 途径。
BMC Cancer. 2024 May 21;24(1):616. doi: 10.1186/s12885-024-12368-6.
6
Engineering 5-flourouracil and leucovorin-loaded vesicular systems for possible colon specific delivery: evaluation and real time cell assay against HCT-116 colon cell lines.构建负载5-氟尿嘧啶和亚叶酸的囊泡系统用于可能的结肠特异性递送：针对HCT-116结肠癌细胞系的评估及实时细胞分析
Heliyon. 2024 Apr 13;10(8):e28872. doi: 10.1016/j.heliyon.2024.e28872. eCollection 2024 Apr 30.
7
Asparagine and Glutamine Deprivation Alters Ionizing Radiation Response, Migration and Adhesion of a p53 Colorectal Cancer Cell Line.天冬酰胺和谷氨酰胺缺乏改变了 p53 结直肠癌细胞系对电离辐射的反应、迁移和黏附。
Int J Mol Sci. 2023 Feb 3;24(3):2983. doi: 10.3390/ijms24032983.
8
Aptamers for Addressed Boron Delivery in BNCT: Effect of Boron Cluster Attachment Site on Functional Activity.针对 BNCT 中硼递呈的适配体：硼簇连接位点对功能活性的影响。
Int J Mol Sci. 2022 Dec 24;24(1):306. doi: 10.3390/ijms24010306.
9
The effect of noisome preparation methods in encapsulating 5-fluorouracil and real time cell assay against HCT-116 colon cancer cell line.恶臭制剂方法对5-氟尿嘧啶的包封效果及对HCT-116结肠癌细胞系的实时细胞分析。
Heliyon. 2022 Dec 16;8(12):e12369. doi: 10.1016/j.heliyon.2022.e12369. eCollection 2022 Dec.
10
Application of advanced technology in traditional Chinese medicine for cancer therapy.先进技术在中医癌症治疗中的应用。
Front Pharmacol. 2022 Oct 13;13:1038063. doi: 10.3389/fphar.2022.1038063. eCollection 2022.
Flavones inhibit breast cancer proliferation through the Akt/FOXO3a signaling pathway.
黄酮类化合物通过Akt/FOXO3a信号通路抑制乳腺癌细胞增殖。
BMC Cancer. 2015 Dec 16;15:958. doi: 10.1186/s12885-015-1965-7.
4
Effect of aluminium on migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells in culture.铝对培养的雌激素无反应性MDA-MB-231人乳腺癌细胞迁移的影响。
J Inorg Biochem. 2015 Nov;152:180-5. doi: 10.1016/j.jinorgbio.2015.09.001. Epub 2015 Sep 8.
5
Anti-growth effect of a novel trans-dichloridobis[2-(2-hydroxyethyl)pyridine]platinum (II) complex via induction of apoptosis on breast cancer cell lines.新型反式二氯双[2-(2-羟乙基)吡啶]铂(II)配合物通过诱导凋亡对乳腺癌细胞系的抗增殖作用
Bioorg Med Chem. 2015 Aug 1;23(15):4303-4310. doi: 10.1016/j.bmc.2015.06.037. Epub 2015 Jun 19.
6
Real-time cell analysis of the inhibitory effect of vitamin K2 on adhesion and proliferation of breast cancer cells.维生素K2对乳腺癌细胞黏附和增殖抑制作用的实时细胞分析
Nutr Res. 2015 Aug;35(8):736-43. doi: 10.1016/j.nutres.2015.05.014. Epub 2015 May 30.
7
Lifestyle changes for prevention of breast cancer.预防乳腺癌的生活方式改变。
Electron Physician. 2014 Jul 1;6(3):894-905. doi: 10.14661/2014.894-905. eCollection 2014 Jul-Sep.
8
Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bioactive constituents in the crude extract.番木瓜核提取物对人乳腺癌（MCF-7 和 MDA-MB-231 细胞系）的细胞毒性作用及粗提取物中的生物活性成分。
BMC Complement Altern Med. 2014 Jun 25;14:199. doi: 10.1186/1472-6882-14-199.
9
xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies.用于实时无标记监测血液系统恶性肿瘤细胞系生长和活力的xCELLigence系统。
Onco Targets Ther. 2014 Jun 12;7:985-94. doi: 10.2147/OTT.S62887. eCollection 2014.
10
Assessment of ovarian cancer spheroid attachment and invasion of mesothelial cells in real time.实时评估卵巢癌球体对间皮细胞的附着和侵袭。
J Vis Exp. 2014 May 20(87):51655. doi: 10.3791/51655.