State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China.
State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China; Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, College of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
Bioresour Technol. 2014 Mar;155:144-51. doi: 10.1016/j.biortech.2013.12.012. Epub 2013 Dec 11.
The mutual interactions among the consortium constructed by four indigenous bacteria and five inter-kingdom fusants and the effects of nitrogen and carbon supplementations on lignin degradation and laccase activity were investigated. Analyzed by Plackett-Burman and central composite design, the microbial consortium were optimized, Bacillus sp. (B) and PE-9 and Pseudomonas putida (Pp) and PE-9 had significant interactions on lignin degradation based on a 5% level of significance. The nitrogen and carbon supplementations played an important role in lignin degradation and laccase production. The ultimate lignin degradation efficiency of 96.0% and laccase activity of 268U/L were obtained with 0.5g/L of ammonium chloride and 2g/L of sucrose. Results suggested that a stable and effective microbial consortium in alkalescent conditions was successfully achieved through the introduction of fusants, which was significant for its industrial application.
研究了由 4 种土著细菌和 5 种跨界融合体构建的联合体之间的相互作用，以及氮、碳补充对木质素降解和漆酶活性的影响。通过 Plackett-Burman 和中心组合设计分析，对微生物联合体进行了优化，基于 5%的显著性水平，芽孢杆菌（B）和 PE-9 以及铜绿假单胞菌（Pp）和 PE-9 对木质素降解有显著的相互作用。氮、碳补充对木质素降解和漆酶生产起着重要作用。在 0.5g/L 氯化铵和 2g/L 蔗糖的条件下，木质素降解的最终效率达到 96.0%，漆酶活性达到 268U/L。结果表明，通过引入融合体成功地在碱性条件下获得了稳定有效的微生物联合体，这对其工业应用具有重要意义。
