König W, Faltin Y, Scheffer J, Schöffler H, Braun V
Lehrstuhl für Medizinische Mikrobiologie und Immunologie, Ruhr-Universität Bochum, Federal Republic of Germany.
Infect Immun. 1987 Nov;55(11):2554-61. doi: 10.1128/iai.55.11.2554-2561.1987.
The hemolytic activities of clinical isolates of Serratia marcescens, of Serratia liquefaciens, and of Escherichia coli strains containing a cloned hemolysin gene of S. marcescens were determined. Hemolysis was induced only by cells and not by spent media. The hemolytically active bacteria induced the release of the leukotriene C4 and of much less leukotriene B4 from polymorphonuclear leukocytes, the release of histamine from rat mast cells, and chemoluminescence of neutrophils. The hemolytic activity was correlated with the response of the leukocytes, but quantitative differences were recorded with regard to the release of the inflammatory mediators. Therefore, other factors in addition to the hemolysin contribute to the stimulation of leukotriene generation and histamine release. It is concluded that the hemolysin via these inflammatory mediators can increase vascular permeability, edema formation, and granulocyte accumulation and thus contributes to the pathogenicity of Serratia species.
测定了粘质沙雷氏菌、液化沙雷氏菌临床分离株以及含有粘质沙雷氏菌克隆溶血素基因的大肠杆菌菌株的溶血活性。溶血仅由细胞诱导，而非用过的培养基诱导。具有溶血活性的细菌可诱导多形核白细胞释放白三烯C4和少量白三烯B4，诱导大鼠肥大细胞释放组胺，并诱导中性粒细胞产生化学发光。溶血活性与白细胞的反应相关，但在炎症介质释放方面记录到了定量差异。因此，除溶血素外的其他因素也有助于刺激白三烯生成和组胺释放。得出的结论是，溶血素通过这些炎症介质可增加血管通透性、水肿形成和粒细胞聚集，从而有助于沙雷氏菌属的致病性。
