玻璃体内注射后的反流体积与成分
Volume and composition of reflux after intravitreal injection.
作者信息
Brodie Frank L, Ruggiero Jason, Ghodasra Devon H, Hui James Z, VanderBeek Brian L, Brucker Alexander J
机构信息
*Scheie Eye Institute, Department of Ophthalmology, Perelman School of Medicine, †Penn HHMI-NIBIB Interface Program in Biomedical Imaging, Department of Bioengineering, and ‡Center for Clinical Epidemiology and Biostatistics, Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
出版信息
Retina. 2014 Jul;34(7):1473-6. doi: 10.1097/IAE.0000000000000098.
PURPOSE
To quantify the amount of drug loss from cadaveric human eyes, which are injected via the pars plana with a known volume of dye at variable intraocular pressures.
METHODS
Eight cadaver eyes were divided into 2 intraocular pressure groups: normal (15 mmHg; 4 eyes) or high (30 mmHg; 4 eyes). Each eye was injected with 50 μL of hematoxylin dye, and the subsequent reflux was immediately collected on a Schirmer's test strip. The test strip was scanned and digitally analyzed to determine the area of saturation and total color intensity present. Using a previously established equation, total volume of reflux and the amount of dye within that reflux were calculated.
RESULTS
The average total volume of refluxed fluid was 1.68 μL (median, 0.62 μL), with a range of 0 μL to 8.05 μL. The average volume of refluxed dye was 0.37 μL (median, 0.08 μL), with a range of 0 μL to 2.15 μL. On average, only 0.74% of the original 50 μL of injected dye was lost (median, 0.15%), with a range from 0% to 4.30%.
CONCLUSION
Although the presence of subconjunctival bleb formation after intravitreal injection may be a concern to the clinician, data from the present study shows that only a very small amount of the injected therapeutic agent is lost in the reflux.
目的
量化在不同眼内压下经睫状体扁平部向尸体人眼注射已知体积染料后药物损失的量。
方法
将8只尸体眼分为2个眼内压组：正常眼内压组（15 mmHg；4只眼）和高眼内压组（30 mmHg；4只眼）。每只眼注射50 μL苏木精染料，随后立即用泪液试纸收集反流液。对试纸进行扫描并进行数字分析，以确定饱和面积和总颜色强度。使用先前建立的公式，计算反流的总体积和该反流中染料的量。
结果
反流液的平均总体积为1.68 μL（中位数为0.62 μL），范围为0 μL至8.05 μL。反流染料的平均体积为0.37 μL（中位数为0.08 μL），范围为0 μL至2.15 μL。平均而言，最初注射的50 μL染料中仅损失0.74%（中位数为0.15%），范围为0%至4.30%。
结论
尽管玻璃体内注射后结膜下形成水泡可能是临床医生关注的问题，但本研究数据表明，反流中仅损失极少量的注射治疗剂。
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