人类三原核胚胎中卵裂模式的改变及其与受精方法的关联：一项延时研究。
Altered cleavage patterns in human tripronuclear embryos and their association to fertilization method: a time-lapse study.
作者信息
Joergensen Mette Warming, Agerholm Inge, Hindkjaer Johnny, Bolund Lars, Sunde Lone, Ingerslev Hans Jakob, Kirkegaard Kirstine
机构信息
Department of Clinical Genetics, Vejle Hospital, Kappeltoft 25, 7100, Vejle, Denmark,
出版信息
J Assist Reprod Genet. 2014 Apr;31(4):435-42. doi: 10.1007/s10815-014-0178-3. Epub 2014 Jan 24.
PURPOSE
To analyze the cleavage patterns in dipronuclear (2PN) and tripronuclear (3PN) embryos in relation to fertilization method.
METHOD
Time-lapse analysis.
RESULTS
Compared to 2PN, more 3PN IVF embryos displayed early cleavage into 3 cells (p < 0.001), displayed longer duration of the 3-cell stage (p < 0.001), and arrested development from the compaction stage and onwards (p < 0.001). For the IVF embryos, the 2nd and 3rd cleavage cycles were completed within the expected time frame. However, timing of the cell divisions within the cleavage cycles differed between the two groups. In contrast, the completion of the 1st, 2nd, and 3rd cleavage cycle was delayed, but with a similar division pattern for 3PN ICSI compared with the 2PN ICSI embryos. 3PN, more often than 2PN ICSI embryos, displayed early cleavage into 3 cells (p = 0.03) and arrested development from the compaction stage and onwards (p = 0.001). More 3PN IVF than ICSI embryos displayed early cleavage into 3 cells (p < 0.001).
CONCLUSIONS
This study reports differences in cleavage patterns between 2PN and 3PN embryos and for the first time demonstrates differences in the cleavage pattern between 3PN IVF and ICSI embryos.
目的
分析双原核（2PN）和三原核（3PN）胚胎的卵裂模式与受精方式的关系。
方法
延时分析。
结果
与2PN胚胎相比，更多的3PN体外受精（IVF）胚胎显示早期卵裂为3细胞（p<0.001），3细胞期持续时间更长（p<0.001），并从致密化阶段及之后停止发育（p<0.001）。对于IVF胚胎，第2和第3次卵裂周期在预期时间内完成。然而，两组之间卵裂周期内细胞分裂的时间不同。相比之下，3PN卵胞浆内单精子注射（ICSI）胚胎的第1、2和第3次卵裂周期完成延迟，但与2PN ICSI胚胎的分裂模式相似。与2PN ICSI胚胎相比，3PN胚胎更常显示早期卵裂为3细胞（p=0.03），并从致密化阶段及之后停止发育（p=0.001）。3PN IVF胚胎比ICSI胚胎更常显示早期卵裂为3细胞（p<0.001）。
结论
本研究报告了2PN和3PN胚胎之间卵裂模式的差异，并首次证明了3PN IVF和ICSI胚胎之间卵裂模式的差异。
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