对来自墨西哥的梨火疫病菌株进行全基因组测序，发现核糖体蛋白S12基因（rpsL）中的单核苷酸多态性赋予链霉素抗性，以及无毒蛋白Rpt2效应子中的单核苷酸多态性改变了宿主互作。
Whole-Genome Sequencing of Erwinia amylovora Strains from Mexico Detects Single Nucleotide Polymorphisms in rpsL Conferring Streptomycin Resistance and in the avrRpt2 Effector Altering Host Interactions.
作者信息
Smits Theo H M, Guerrero-Prieto Víctor M, Hernández-Escarcega Germán, Blom Jochem, Goesmann Alexander, Rezzonico Fabio, Duffy Brion, Stockwell Virginia O
机构信息
Research Group for Environmental Genomics and Systems Biology, Institute for Natural Resources Sciences, Zurich University of Applied Sciences ZHAW, Wädenswil, Switzerland.
出版信息
Genome Announc. 2014 Jan 23;2(1):e01229-13. doi: 10.1128/genomeA.01229-13.
Abstract
We report draft genome sequences of three Mexican Erwinia amylovora strains. A novel plasmid, pEA78, was identified. Comparative genomics revealed an rpsL chromosomal mutation conferring high-level streptomycin resistance in two strains. In the effector gene avrRpt2, a single nucleotide polymorphism was detected that overcomes fire blight disease resistance in Malus × robusta 5.
摘要
我们报告了三株墨西哥梨火疫病菌株的基因组草图序列。鉴定出一种新型质粒pEA78。比较基因组学揭示了在两株菌株中存在一个导致高水平链霉素抗性的rpsL染色体突变。在效应子基因avrRpt2中，检测到一个单核苷酸多态性，该多态性可克服苹果×鲁斯塔5号对火疫病的抗性。
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