Department of Pharmacology, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune 411041, India; E-mail:
Department of Pharmacology, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune 411041, India.
J Integr Med. 2014 Jan;12(1):35-41. doi: 10.1016/S2095-4964(14)60001-7.
The present study was undertaken to evaluate the effect of diosmin in diabetic neuropathy in type 2 diabetic rats.
Type 2 diabetes was induced in male Sprague-Dawley rats by single intraperitoneal injection of streptozotocin (35 mg/kg) and high-fat diet. Four weeks after the confirmation of diabetes, diabetic rats were treated with diosmin (50 and 100 mg/kg, p.o.) for next 4 weeks. Rats were evaluated for biochemical, behavioral and oxidative stress parameters. Eddy's hot plate and tail immersion test were performed on 6th, 7th, 8th, 9th and 10th weeks of experiment to assess thermal hyperalgesia and cold allodynia respectively. Further, the walking function test was performed for assessing the motor responses at the end of the treatment schedule.
Rats were fed with high-fat diet throughout the experiment schedule and administration of low-dose streptozotocin induced significant elevation in blood glucose level and insulin resistance which was confirmed by oral glucose tolerance test. Treatment with diosmin at doses of 50 and 100 mg/kg significantly restored the reduced body weight, elevated blood sugar and lipid profiles. Further the dose-dependent improvement was observed in thermal hyperalgesia, cold allodynia and walking function in diabetic rats treated with diosmin. Elevated levels of malondialdehyde, and nitric oxide and decreased glutathione levels and superoxide dismutase activity in diabetic rats were restored significantly after the 4 weeks of diosmin treatment.
Diosmin has shown beneficial effect in preventing the progression of early diabetic neuropathy in rats.
本研究旨在评估地奥司明在 2 型糖尿病大鼠糖尿病神经病变中的作用。
雄性 Sprague-Dawley 大鼠一次性腹腔注射链脲佐菌素（35mg/kg）和高脂饮食诱导 2 型糖尿病。糖尿病确诊 4 周后，地奥司明（50 和 100mg/kg，po）治疗 4 周。评估大鼠生化、行为和氧化应激参数。在实验的第 6、7、8、9 和 10 周进行 Eddy 热板和尾巴浸浴试验，分别评估热痛觉过敏和冷感觉异常。进一步在治疗方案结束时进行行走功能试验，以评估运动反应。
大鼠在整个实验过程中给予高脂饮食，低剂量链脲佐菌素给药导致血糖水平和胰岛素抵抗显著升高，口服葡萄糖耐量试验证实了这一点。地奥司明 50 和 100mg/kg 剂量治疗显著恢复了降低的体重、升高的血糖和血脂谱。进一步观察到，地奥司明治疗的糖尿病大鼠的热痛觉过敏、冷感觉异常和行走功能得到剂量依赖性改善。糖尿病大鼠的丙二醛、一氧化氮水平升高，谷胱甘肽水平和超氧化物歧化酶活性降低，经 4 周地奥司明治疗后显著恢复。
地奥司明在预防大鼠早期糖尿病神经病变进展方面显示出有益的效果。
