Kwon Jee Young, Lee Seung Young, Koedrith Preeyaporn, Lee Jong Yun, Kim Kyoung-Min, Oh Jae-Min, Yang Sung Ik, Kim Meyoung-Kon, Lee Jong Kwon, Jeong Jayoung, Maeng Eun Ho, Lee Beam Jun, Seo Young Rok
Department of Life Science, Institute of Environmental Medicine, Dongguk University, Seoul, South Korea; Department of Biomedical Science, School of Medicine, Kyung Hee University, Seoul, South Korea.
Korea Testing and Research Institute, Seoul, South Korea; College of Veterinary Medicine Chungbuk National University, Cheongju, Chungcheongbuk-do, South Korea.
Mutat Res Genet Toxicol Environ Mutagen. 2014 Feb;761:1-9. doi: 10.1016/j.mrgentox.2014.01.005. Epub 2014 Jan 22.
The industrial application of nanotechnology, particularly using zinc oxide (ZnO), has grown rapidly, including products such as cosmetics, food, rubber, paints, and plastics. However, despite increasing population exposure to ZnO, its potential genotoxicity remains controversial. The biological effects of nanoparticles depend on their physicochemical properties. Preparations with well-defined physico-chemical properties and standardized test methods are required for assessing the genotoxicity of nanoparticles. In this study, we have evaluated the genotoxicity of four kinds of ZnO nanoparticles: 20nm and 70nm size, positively or negatively charged. Four different genotoxicity tests (bacterial mutagenicity assay, in vitro chromosomal aberration test, in vivo comet assay, and in vivo micronucleus test, were conducted, following Organization for Economic Cooperation and Development (OECD) test guidelines with good laboratory practice (GLP) procedures. No statistically significant differences from the solvent controls were observed. These results suggest that surface-modified ZnO nanoparticles do not induce genotoxicity in in vitro or in vivo test systems.
纳米技术的工业应用，尤其是使用氧化锌（ZnO）的应用，发展迅速，涵盖了化妆品、食品、橡胶、涂料和塑料等产品。然而，尽管越来越多的人接触到ZnO，但其潜在的遗传毒性仍存在争议。纳米颗粒的生物学效应取决于其物理化学性质。评估纳米颗粒的遗传毒性需要具有明确物理化学性质的制剂和标准化的测试方法。在本研究中，我们评估了四种ZnO纳米颗粒的遗传毒性：20nm和70nm大小，带正电或带负电。按照经济合作与发展组织（OECD）的测试指南并采用良好实验室规范（GLP）程序，进行了四种不同的遗传毒性测试（细菌致突变性试验、体外染色体畸变试验、体内彗星试验和体内微核试验）。未观察到与溶剂对照有统计学显著差异。这些结果表明，表面改性的ZnO纳米颗粒在体外或体内测试系统中不会诱导遗传毒性。
