洋甘菊（Matricaria recutita L.）汤提取物在大鼠中的止泻和抗氧化活性。
Antidiarrheal and antioxidant activities of chamomile (Matricaria recutita L.) decoction extract in rats.
机构信息
Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia; Laboratoire de Nutrition et Physiologie Animale, Institut Supérieur de Biotechnologie de Béja, Avenue Habib Bourguiba, B.P., 382-9000 Béja, Tunisia.
Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia; Laboratoire de Nutrition et Physiologie Animale, Institut Supérieur de Biotechnologie de Béja, Avenue Habib Bourguiba, B.P., 382-9000 Béja, Tunisia.
出版信息
J Ethnopharmacol. 2014 Mar 14;152(2):327-32. doi: 10.1016/j.jep.2014.01.015. Epub 2014 Jan 22.
ETHNOPHARMACOLOGICAL RELEVANCE
Matricaria recutita L. (Chamomile) has been widely used in the Tunisian traditional medicine for the treatment of digestive system disorders. The present work aims to investigate the protective effects of chamomile decoction extract (CDE) against castor oil-induced diarrhea and oxidative stress in rats.
METHODS
The antidiarrheal activity was evaluated using castor oil-induced diarrhea method. In this respect, rats were divided into six groups: Control, Castor oil, Castor oil+Loperamide (LOP) and Castor oil+various doses of CDE. Animals were per orally (p.o.) pre-treated with CDE during 1h and intoxicated for 2 or 4h by acute oral administration of castor oil.
RESULTS
Our results showed that CDE produced a significant dose-dependent protection against castor oil-induced diarrhea and intestinal fluid accumulation. On the other hand, we showed that diarrhea was accompagned by an oxidative stress status assessed by an increase of malondialdehyde (MDA) level and depletion of antioxidant enzyme activities as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Castor oil also increased gastric and intestinal mucosa hydrogen peroxide (H2O2) and free iron levels. Importantly, we showed that chamomile pre-treatment abrogated all these biochemical alterations.
CONCLUSION
These findings suggested that chamomile extract had a potent antidiarrheal and antioxidant properties in rats confirming their use in traditional medicine.
民族药理学相关性
母菊（Chamomile）在突尼斯传统医学中被广泛用于治疗消化系统疾病。本研究旨在探讨母菊汤提取物（CDE）对蓖麻油诱导的腹泻和大鼠氧化应激的保护作用。
方法
采用蓖麻油诱导腹泻法评价止泻活性。为此，将大鼠分为六组：对照组、蓖麻油组、蓖麻油+洛哌丁胺（LOP）组和蓖麻油+不同剂量 CDE 组。动物在急性口服蓖麻油前 1h 经口（p.o.）给予 CDE 预处理，持续 2 或 4h。
结果
结果表明，CDE 对蓖麻油诱导的腹泻和肠液积聚具有显著的剂量依赖性保护作用。另一方面，我们表明腹泻伴随着氧化应激状态，表现为丙二醛（MDA）水平升高和超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）等抗氧化酶活性降低。蓖麻油还增加了胃和肠黏膜的过氧化氢（H2O2）和游离铁水平。重要的是，我们表明母菊预处理消除了所有这些生化变化。
结论
这些发现表明，母菊提取物对大鼠具有较强的止泻和抗氧化作用，证实了其在传统医学中的应用。
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