Unité de Physiologie Fonctionnelle et Valorisation des Bio-Ressources, Institut Supérieur de Biotechnologie de Beja, Université de Jendouba, Beja, Tunisia.
Laboratoire des Ressources Sylvo-Pastorales, Institut Sylvo-Pastoral de Tabarka, Université de Jendouba, Tabarka, Tunisia.
Neurogastroenterol Motil. 2021 Jun;33(6):e14065. doi: 10.1111/nmo.14065. Epub 2020 Dec 15.
Diarrhea is a multifactorial gastrointestinal disorder responsible for about 5 million deaths annually. The chemical composition, the antioxidant activity of Crataegus azarolus berries aqueous extract (CABAE) as well as its protective effects against castor oil-induced diarrhea, oxidative stress, and inflammation in rat were studied.
Sixty male rats were used and divided into six groups of ten animals in each: Control (C), castor oil (CO), CO+various doses of CABAE (100, 200, and 400 mg/kg b.w., p.o.), and CO+loperamide (LOP, 10 mg/kg b.w., p.o.).
The CABAE showed relatively high levels of total polyphenols, flavonoids, and tannins. The LC-HRESIMS technique allowed the identification of 5 phenolic compounds and the major component is quinic acid. In vivo studies showed that CABAE protected against castor oil-induced diarrhea and intestinal fluid accumulation. The CABAE counteracted castor oil-induced lipoperoxidation, preserved GSH and thiol groups levels, and prevented the depletion of antioxidant enzyme activities, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). The CABAE administration also protected against castor oil-induced inflammatory markers (ALP and CRP) increase. More importantly, castor oil induced an increase of intracellular mediators, such as hydrogen peroxide, free iron, and calcium, while CABAE pretreatment significantly reversed them to near control levels.
The Crataegus azarolus berries aqueous extract significantly protected against diarrhea due in part to its antioxidant and anti-inflammatory properties.
腹泻是一种多因素的胃肠道紊乱疾病，每年导致约 500 万人死亡。本研究旨在探讨山楂浆果水提物（CABAE）的化学成分、抗氧化活性，以及其对蓖麻油诱导的腹泻、氧化应激和炎症的保护作用。
将 60 只雄性大鼠分为 6 组，每组 10 只：对照组（C）、蓖麻油组（CO）、CO+不同剂量 CABAE（100、200 和 400mg/kg b.w.，p.o.）和 CO+洛哌丁胺组（LOP，10mg/kg b.w.，p.o.）。
CABAE 表现出相对较高水平的总多酚、类黄酮和单宁。LC-HRESIMS 技术允许鉴定出 5 种酚类化合物，主要成分是奎尼酸。体内研究表明，CABAE 可预防蓖麻油诱导的腹泻和肠液积聚。CABAE 可对抗蓖麻油诱导的脂质过氧化，维持 GSH 和巯基水平，并防止抗氧化酶活性（如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx））的耗竭。CABAE 给药还可防止蓖麻油诱导的炎症标志物（ALP 和 CRP）升高。更重要的是，蓖麻油诱导细胞内介质（如过氧化氢、游离铁和钙）增加，而 CABAE 预处理可显著将其逆转至接近对照水平。
山楂浆果水提物可显著预防腹泻，部分原因是其具有抗氧化和抗炎特性。
