Umnova N S, Zheludkov M M, Pavlova I P, Shakhanina K L
Zh Mikrobiol Epidemiol Immunobiol. 1987 Sep(9):103-7.
The conditions of the enzyme immunoassay for the detection of Brucella antigens have been selected, making it possible to detect these antigens both in solutions and in biological material within 3-4 hours. In guinea pigs infected with B. abortus 99 in a dose of 1,000 microbial cells, brucellar antigen has been detectable in the organs and blood serum of the animals as early as 24 hours after infection. This assay, if carried out under the optimal conditions, detects soluble brucellar polysaccharide antigen at a concentration of 1 ng/ml and Brucellae at a concentration of 5 X 10(4) microbial cells/ml in the presence of 200-fold surplus of other bacterial cells.
已筛选出用于检测布鲁氏菌抗原的酶免疫测定条件，从而能够在3 - 4小时内检测溶液和生物材料中的这些抗原。在用1000个微生物细胞剂量的流产布鲁氏菌99株感染的豚鼠中，早在感染后24小时就能在动物的器官和血清中检测到布鲁氏菌抗原。在最佳条件下进行此测定时，在存在200倍过量其他细菌细胞的情况下，可检测到浓度为1 ng/ml的可溶性布鲁氏菌多糖抗原以及浓度为5×10⁴个微生物细胞/ml的布鲁氏菌。
