Pilch Wanda, Szygula Zbigniew, Tyka Anna K, Palka Tomasz, Tyka Aleksander, Cison Tomasz, Pilch Pawel, Teleglow Aneta
Department of Physiology and Biochemistry, University School of Physical Education, Cracow, Poland.
Department of Sports Medicine, University School of Physical Education, Cracow, Poland.
PLoS One. 2014 Jan 20;9(1):e85320. doi: 10.1371/journal.pone.0085320. eCollection 2014.
The aim of the study was to investigate pro-oxidant-antioxidant balance in two series of examinations with two types of stressors (exogenous heat and the combined exogenous and endogenous heat) in trained and untrained men. The exogenous stressor was provided by Finnish sauna session, whereas the combined stressor was represented by the exercise in elevated ambient temperature. The men from the two groups performed the physical exercise on a cycle ergometer with the load of 53 ± 2% maximal oxygen uptake at the temperature of 33 ± 1 °C and relative humidity of 70% until their rectal temperature rose by 1.2 °C. After a month from completion of the exercise test the subjects participated in a sauna bathing session with the temperature of 96 ± 2 °C, and relative humidity of 16 ± 5%. 15-minutes heating and 2-minute cool-down in a shower with the temperature of 20 °C was repeated until rectal temperature rose by 1.2 °C compared to the initial value. During both series of tests rectal temperature was measured at 5-minute intervals. Before both series of tests and after them body mass was measured and blood samples were taken for biochemical tests. Serum total protein, serum concentration of lipid peroxidation products and serum antioxidants were determined. The athletes were characterized by higher level of antioxidant status and lower concentration of lipid peroxidation products. Physical exercise at elevated ambient temperature caused lower changes in oxidative stress indices compared to sauna bathing. Sauna induced a shift in pro-oxidant-antioxidant balance towards oxidation, which was observed less intensively in the athletes compared to the untrained men. This leads to the conclusion that physical exercise increases tolerance to elevated ambient temperature and oxidative stress.
该研究的目的是调查在两组测试中，训练有素和未经训练的男性在面对两种应激源（外源性热以及外源性和内源性热的组合）时的促氧化剂-抗氧化剂平衡。外源性应激源由芬兰桑拿浴提供，而组合应激源则由在升高的环境温度下进行运动来体现。两组男性在温度为33±1°C、相对湿度为70%的条件下，以最大摄氧量的53±2%的负荷在自行车测力计上进行体育锻炼，直到直肠温度升高1.2°C。在运动测试完成一个月后，受试者参加温度为96±2°C、相对湿度为16±5%的桑拿浴。重复进行15分钟加热和在20°C淋浴中2分钟冷却，直到直肠温度比初始值升高1.2°C。在这两组测试期间，每隔5分钟测量一次直肠温度。在两组测试之前和之后测量体重，并采集血样进行生化测试。测定血清总蛋白、脂质过氧化产物的血清浓度和血清抗氧化剂。运动员的特点是抗氧化状态水平较高，脂质过氧化产物浓度较低。与桑拿浴相比，在升高的环境温度下进行体育锻炼引起的氧化应激指标变化较小。桑拿浴导致促氧化剂-抗氧化剂平衡向氧化方向转变，与未训练的男性相比，在运动员中观察到的这种转变强度较小。由此得出结论，体育锻炼可提高对升高的环境温度和氧化应激的耐受性。
