Moody Matthew J, Jones Stephanie E, Elliot Marie A
Department of Biology and Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
PLoS One. 2014 Jan 20;9(1):e85856. doi: 10.1371/journal.pone.0085856. eCollection 2014.
Streptomyces are predominantly soil-dwelling bacteria that are best known for their multicellular life cycle and their prodigious metabolic capabilities. They are also renowned for their regulatory capacity and flexibility, with each species encoding >60 sigma factors, a multitude of transcription factors, and an increasing number of small regulatory RNAs. Here, we describe our characterization of a conserved small RNA (sRNA), scr4677. In the model species Streptomyces coelicolor, this sRNA is located in the intergenic region separating SCO4677 (an anti-sigma factor-encoding gene) and SCO4676 (a putative regulatory protein-encoding gene), close to the SCO4676 translation start site in an antisense orientation. There appears to be considerable genetic interplay between these different gene products, with wild type expression of scr4677 requiring function of the anti-sigma factor SCO4677, and scr4677 in turn influencing the abundance of SCO4676-associated transcripts. The scr4677-mediated effects were independent of RNase III (a double stranded RNA-specific nuclease), with RNase III having an unexpectedly positive influence on the level of SCO4676-associated transcripts. We have shown that both SCO4676 and SCO4677 affect the production of the blue-pigmented antibiotic actinorhodin under specific growth conditions, and that this activity appears to be independent of scr4677.
链霉菌主要是生活在土壤中的细菌，以其多细胞生命周期和巨大的代谢能力而闻名。它们还因其调控能力和灵活性而著称，每个物种编码超过60种σ因子、大量转录因子以及越来越多的小调控RNA。在此，我们描述了对一种保守的小RNA（sRNA）scr4677的表征。在模式物种天蓝色链霉菌中，这种sRNA位于分隔SCO4677（一个编码抗σ因子的基因）和SCO4676（一个假定的编码调控蛋白的基因）的基因间区域，以反义方向靠近SCO4676的翻译起始位点。这些不同基因产物之间似乎存在相当多的遗传相互作用，scr4677的野生型表达需要抗σ因子SCO4677的功能，而scr4677反过来又影响与SCO4676相关的转录本的丰度。scr4677介导的效应独立于RNase III（一种双链RNA特异性核酸酶），RNase III对与SCO4676相关的转录本水平具有意想不到的正向影响。我们已经表明，SCO4676和SCO4677在特定生长条件下都会影响蓝色色素抗生素放线紫红素的产生，并且这种活性似乎独立于scr4677。
