Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Mol Microbiol. 2010 Feb;75(3):781-91. doi: 10.1111/j.1365-2958.2009.07023.x. Epub 2010 Jan 3.
RNase III family enzymes, which are perhaps the most widely conserved of all ribonucleases, are known primarily for their role in the processing and maturation of small RNAs. The RNase III gene of Streptomyces coelicolor, which was discovered initially as a global regulator of antibiotic production in this developmentally complex bacterial species and named absB (antibiotic biosynthesis gene B), has subsequently also been found to modulate the cellular abundance of multiple messenger RNAs implicated in morphological differentiation. We report here that regulation of differentiation-related mRNAs by the S. coelicolor AbsB/RNase III enzyme occurs largely by ribonucleolytic cleavage of transcripts encoding the pleiotropic transcription factor, AdpA, and that AdpA and AbsB participate in a novel feedback-control loop that reciprocally regulates the cellular levels of both proteins. Our results reveal a previously unsuspected mechanism for global ribonuclease-mediated control of gene expression in streptomycetes.
RNase III 家族酶可能是所有核糖核酸酶中最广泛保守的，其主要作用是参与小 RNA 的加工和成熟。天蓝色链霉菌的 RNase III 基因最初被发现是这种发育复杂的细菌物种中抗生素产生的全局调节剂，并被命名为 absB（抗生素生物合成基因 B），随后也被发现调节与形态分化相关的多个信使 RNA 的细胞丰度。我们在这里报告，由 S. coelicolor AbsB/RNase III 酶调控的分化相关 mRNA 主要通过转录因子 AdpA 的多效性转录物的核糖核酸酶切割发生，并且 AdpA 和 AbsB 参与一种新的反馈控制回路，该回路可相互调节两种蛋白质的细胞水平。我们的研究结果揭示了一种以前未被怀疑的机制，用于放线菌中全局核糖核酸酶介导的基因表达控制。
