产碳青霉烯酶肠杆菌科细菌的快速检测策略。
Strategy for rapid detection of carbapenemase-producing Enterobacteriaceae.
作者信息
Dortet Laurent, Bréchard Ludivine, Cuzon Gaëlle, Poirel Laurent, Nordmann Patrice
机构信息
INSERM U914, South-Paris Medical School, Le Kremlin-Bicêtre, France.
出版信息
Antimicrob Agents Chemother. 2014;58(4):2441-5. doi: 10.1128/AAC.01239-13. Epub 2014 Jan 27.
Abstract
A prospective survey was conducted on 862 Enterobacteriaceae isolates with reduced susceptibility to carbapenems. The Carba NP test, UV spectrophotometry, and a DNA microarray were used to detect carbapenemase producers, and the results were compared to those from PCR and sequencing. The 172 carbapenemase producers were detected using the Carba NP test and UV spectrophotometry, whereas the DNA microarray failed to detect IMI producers. The use of the Carba NP test as a first screening, followed by the use of molecular techniques, has been determined to be an efficient strategy for identifying carbapenemase-producing Enterobacteriaceae.
摘要
对862株对碳青霉烯类药物敏感性降低的肠杆菌科细菌进行了前瞻性调查。采用Carba NP试验、紫外分光光度法和DNA微阵列检测碳青霉烯酶产生菌，并将结果与PCR和测序结果进行比较。使用Carba NP试验和紫外分光光度法检测到172株碳青霉烯酶产生菌，而DNA微阵列未能检测到IMI产生菌。已确定将Carba NP试验作为初次筛查，随后使用分子技术，是鉴定产碳青霉烯酶肠杆菌科细菌的有效策略。
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