Weiner A J, Choo Q L, Wang K S, Govindarajan S, Redeker A G, Gerin J L, Houghton M
Chiron Corporation, Emeryville, California 94608.
J Virol. 1988 Feb;62(2):594-9. doi: 10.1128/JVI.62.2.594-599.1988.
On the basis of the complete nucleotide sequence of the single-stranded, covalently closed circular hepatitis delta virus RNA genome (K.-S. Wang, Q.-L. Choo, A. J. Weiner, J.-H. Ou, R. C. Najarian, R. M. Thayer, G. T. Mullenbach, K. J. Denniston, J. L. Gerin, and M. Houghton, Nature [London] 323:508-514, 1986 [Author's correction, 328:456, 1987]), five long open reading frames (ORFs) encoding polypeptides containing a methionine proximal to the amino terminus were expressed in bacteria. Only polypeptides encoded by the antigenomic ORF5 cross-reacted with antisera obtained from patients with hepatitis delta virus infections. Immunological analysis of viral extracts and the recombinant ORF5 polypeptides synthesized in bacteria and yeast cells revealed that ORF5 encodes the immunogenic epitope(s) shared by both hepatitis delta viral polypeptides p27 delta and p24 delta and probably represents the complete structural gene for p27 delta and p24 delta. We also present evidence that ORF5 encodes the hepatitis delta antigen, an antigen originally found in the nuclei of hepatocytes of infected individuals (M. Rizzetto, M. G. Canese, S. Arico, O. Crivelli, F. Bonino, C. G. Trepo, and G. Verme, Gut 18:997-1003, 1977). A comparison of the primary structure of the predicted hepatitis delta antigen polypeptides with that of the core antigen of the hepatitis B virus shows that these polypeptides are very dissimilar.
基于单链、共价闭合环状丁型肝炎病毒RNA基因组的完整核苷酸序列（K.-S. 王、Q.-L. 朱、A. J. 韦纳、J.-H. 欧、R. C. 纳贾里安、R. M. 塞耶、G. T. 马伦巴赫、K. J. 丹尼斯顿、J. L. 格林和M. 霍顿，《自然》[伦敦]323:508 - 514，1986年[作者更正，328:456，1987年]），在细菌中表达了五个长开放阅读框（ORF），这些阅读框编码在氨基末端附近含有甲硫氨酸的多肽。只有反基因组ORF5编码的多肽与从丁型肝炎病毒感染患者获得的抗血清发生交叉反应。对病毒提取物以及在细菌和酵母细胞中合成的重组ORF5多肽进行的免疫学分析表明，ORF5编码丁型肝炎病毒多肽p27δ和p24δ共有的免疫原性表位，可能代表p27δ和p24δ的完整结构基因。我们还提供证据表明，ORF5编码丁型肝炎抗原，该抗原最初在受感染个体的肝细胞核中发现（M. 里泽托、M. G. 卡内塞、S. 阿里科、O. 克里维利、F. 博尼诺、C. G. 特雷波和G. 韦尔梅，《肠道》18:997 - 1003，1977年）。将预测的丁型肝炎抗原多肽的一级结构与乙型肝炎病毒核心抗原的一级结构进行比较，结果表明这些多肽非常不同。
