Division of Pediatric Infectious Diseases, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Saint Paul, MN 55108, USA.
Viruses. 2014 Jan 27;6(2):448-75. doi: 10.3390/v6020448.
Development of a vaccine against congenital infection with human cytomegalovirus is complicated by the issue of re-infection, with subsequent vertical transmission, in women with pre-conception immunity to the virus. The study of experimental therapeutic prevention of re-infection would ideally be undertaken in a small animal model, such as the guinea pig cytomegalovirus (GPCMV) model, prior to human clinical trials. However, the ability to model re-infection in the GPCMV model has been limited by availability of only one strain of virus, the 22122 strain, isolated in 1957. In this report, we describe the isolation of a new GPCMV strain, the CIDMTR strain. This strain demonstrated morphological characteristics of a typical Herpesvirinae by electron microscopy. Illumina and PacBio sequencing demonstrated a genome of 232,778 nt. Novel open reading frames ORFs not found in reference strain 22122 included an additional MHC Class I homolog near the right genome terminus. The CIDMTR strain was capable of dissemination in immune compromised guinea pigs, and was found to be capable of congenital transmission in GPCMV-immune dams previously infected with salivary gland‑adapted strain 22122 virus. The availability of a new GPCMV strain should facilitate study of re-infection in this small animal model.
针对人类巨细胞病毒先天性感染的疫苗的研发受到一个问题的阻碍，即对于病毒有预先免疫的孕妇可能会再次感染，随后发生垂直传播。在进行人体临床试验之前，对实验性治疗性预防再感染的研究理想情况下应在小动物模型中进行，如豚鼠巨细胞病毒（GPCMV）模型。然而，在 GPCMV 模型中模拟再感染的能力受到限制，因为仅有一种病毒株，即 1957 年分离的 22122 株病毒可用。在本报告中，我们描述了一种新的 GPCMV 株的分离，即 CIDMTR 株。该毒株通过电子显微镜显示出典型的疱疹病毒科的形态特征。Illumina 和 PacBio 测序表明基因组大小为 232778nt。在参考株 22122 中未发现的新开放阅读框（ORFs）包括在右基因组末端附近的另一个 MHC 类 I 同源物。CIDMTR 株能够在免疫受损的豚鼠中传播，并能够在先前感染唾液腺适应株 22122 病毒的 GPCMV 免疫母鼠中发生先天性传播。新 GPCMV 株的出现应有助于在该小动物模型中研究再感染。
