Singh Manish K, Dwivedi Shailendra, Yadav Suraj S, Sharma Praveen, Khattri Sanjay
Department of Pharmacology, King George's Medical University, Lucknow, 226 003 India.
Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, 342005 India.
Indian J Clin Biochem. 2014 Jan;29(1):29-37. doi: 10.1007/s12291-013-0353-9. Epub 2013 Jun 15.
Arsenic a metalloid and environmental contaminated has been found to be associated with public health problems in the affected areas. It is naturally occurred in groundwater and its accumulation in plant and animals leads to toxicity in several tissues most notably hepatic organ. Arsenic exposures (3 mg/kg body weight/day for 30 days) in mice exhibited increased arsenic and Zn levels in hepatocytes associated with enhanced oxidative stress in hepatocytes while there were no significantly changes were observed in Cu level. An increase in the lipid peroxidation and decrease in the levels of reduced glutathione and activity of superoxide dismutase, catalase, and glutathione peroxidase were observed in arsenic treated mice as compared to controls. Arsenic exposure in mice also caused a significant change in serum biomarkers in the SGOT, SGPT and creatinine as compared to the controls. There were no significant changes in the serum levels of total protein in these mice. Co-administration of arsenic and fruit extract of amla (500 mg/kg body weight/day for 30 days) caused a significant reduction of arsenic transference associated with significantly decreases hepatic arsenic levels and balanced the antioxidant enzyme and levels of serum hepatic enzymes like SGOT and SGPT. The results of the present study clearly demonstrate the antioxidant property of amla that could be responsible for its protective efficacy in arsenic induced hepatic toxicity.
砷是一种类金属，存在于受污染的环境中，已发现其与受影响地区的公共卫生问题有关。它天然存在于地下水中，在动植物体内的积累会导致多个组织中毒，最显著的是肝脏器官。给小鼠砷暴露（3毫克/千克体重/天，持续30天）后，肝细胞中的砷和锌水平升高，同时肝细胞中的氧化应激增强，而铜水平未观察到显著变化。与对照组相比，砷处理的小鼠中脂质过氧化增加，还原型谷胱甘肽水平降低，超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性降低。与对照组相比，小鼠砷暴露还导致血清中谷草转氨酶、谷丙转氨酶和肌酐等生物标志物发生显著变化。这些小鼠的血清总蛋白水平没有显著变化。同时给予砷和余甘子果实提取物（500毫克/千克体重/天，持续30天）可显著降低砷的转移，同时肝脏砷水平显著降低，并使抗氧化酶和血清肝酶如谷草转氨酶和谷丙转氨酶的水平恢复平衡。本研究结果清楚地证明了余甘子的抗氧化特性，这可能是其对砷诱导的肝毒性具有保护作用的原因。
