对由2,4-二氯苯氧乙酸丁酯处理过的鸡蛋孵化出的雏鸡组织中的核酸含量及残留量的测定。

Nucleic acid content and residue determination in tissues of chicks born from 2,4-dichlorophenoxyacetic butyl ester treated eggs.

作者信息

Duffard R O, Fabra de Peretti A I, Castro de Cantarini S M, Mori de Moro G B, Argüello J M, Evangelista de Duffard A M

机构信息

Department of Molecular Biology, Faculty of Exact, Chemical, Physical and Natural Sciences, National University of Río Cuarto, Córdoba, Argentina.

出版信息

Drug Chem Toxicol. 1987;10(3-4):339-55. doi: 10.3109/01480548709042991.

DOI:10.3109/01480548709042991

PMID:2448113

Abstract

The effects of 2,4-dichlorophenoxyacetic butyl ester (2,4-D b.e.) on protein and nucleic acid contents of different tissues of chicks born from externally treated hen's eggs were studied. Residues of 2,4-D b.e. were also determined and quantified by gas liquid chromatography (GLC). A diminished DNA content was observed in skeletal muscle and stomach, an increased one was found in cerebrum and cerebellum, whereas no variations were observed in the other analysed tissues. Protein levels were found decreased in kidney, cerebrum and heart. Besides, the GLC determinations revealed the presence of the 2,4-D b.e. in all the studied tissues finding the highest concentrations in kidney. 2,4-D did not appear to be a significant metabolite of 2,4-D b.e. in this experimental system. The dissimilar effects of 2,4-D b.e. on protein and nucleic acid contents of the studied tissues would be related to a different tissue susceptibility to the drug and/or to drug distribution.

摘要

研究了2,4-二氯苯氧乙酸丁酯（2,4-D丁酯）对经外部处理的母鸡所产雏鸡不同组织中蛋白质和核酸含量的影响。还通过气相色谱法（GLC）对2,4-D丁酯的残留量进行了测定和定量。观察到骨骼肌和胃中的DNA含量减少，大脑和小脑中的DNA含量增加，而在其他分析组织中未观察到变化。发现肾脏、大脑和心脏中的蛋白质水平降低。此外，GLC测定显示在所有研究组织中均存在2,4-D丁酯，其中肾脏中的浓度最高。在该实验系统中，2,4-D似乎不是2,4-D丁酯的重要代谢产物。2,4-D丁酯对所研究组织中蛋白质和核酸含量的不同影响可能与不同组织对该药物的敏感性和/或药物分布有关。