Mori de Moro G B, Duffard R O, Evangelista de Duffard A M
Neurotoxicology. 1985 Fall;6(3):133-7.
Fertilized hens' eggs were externally treated with 2,4-dichlorophenoxyacetic (2,4-D) butyl ester (2.4 mg/egg) before starting incubation. The specific activity of 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase) was diminished in the whole brain of animals born from treated eggs, as compared with controls. Myelin was isolated by ultracentrifugation from the central nervous system. Treatment with 2,4-D butyl ester produced a 65% reduction. There were no statistically significant differences in myelin phospholipids, cerebrosides, sulphatides and gangliosides between control and treated animals. Cholesterol was diminished by 12% in the treated group, while cholesterol esters were not detectable in either myelin fraction. Total myelin proteins were also decreased by 40%, without variations in their pattern. There were changes in the following ratios in the myelin of the treated group: lipid/protein, cholesterol/phospholipids, cholesterol/protein, phospholipids/protein. Collectively these findings indicate that 2,4-D butyl ester produced hypomyelination.
在开始孵化前，对受精的母鸡卵进行了2,4-二氯苯氧乙酸（2,4-D）丁酯（2.4毫克/卵）的外部处理。与对照组相比，经处理的卵孵化出的动物全脑中2',3'-环核苷酸3'-磷酸水解酶（CNPase）的比活性降低。通过超速离心从中枢神经系统分离出髓磷脂。用2,4-D丁酯处理后，髓磷脂减少了65%。对照动物和经处理的动物在髓磷脂磷脂、脑苷脂、硫脂和神经节苷脂方面没有统计学上的显著差异。处理组中胆固醇减少了12%，而在任何一个髓磷脂组分中都未检测到胆固醇酯。总髓磷脂蛋白也减少了40%，其模式没有变化。处理组髓磷脂中的以下比率发生了变化：脂质/蛋白质、胆固醇/磷脂、胆固醇/蛋白质、磷脂/蛋白质。这些发现共同表明，2,4-D丁酯导致了髓鞘形成不足。
