Xing Lianping, Boyce Brendan F
University of Rochester Medical Center, Rochester, NY, USA.
Methods Mol Biol. 2014;1130:307-313. doi: 10.1007/978-1-62703-989-5_23.
Osteoclasts are the only cell type in the body to resorb bone. Osteoclasts play a critical role in physiologic and pathologic bone remodeling. Many genetic mouse models affect the skeleton by regulating osteoclast function directly or indirectly. This protocol describes a procedure for generating osteoclasts from mouse bone marrow cells using macrophage colony stimulating factor and receptor activator of NF-κB ligand.
破骨细胞是体内唯一能够吸收骨组织的细胞类型。破骨细胞在生理性和病理性骨重塑过程中发挥着关键作用。许多基因工程小鼠模型通过直接或间接调节破骨细胞功能来影响骨骼。本实验方案描述了一种使用巨噬细胞集落刺激因子和核因子κB受体活化因子配体从小鼠骨髓细胞生成破骨细胞的方法。
