From the Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033.
J Biol Chem. 2014 Mar 28;289(13):9221-32. doi: 10.1074/jbc.M113.542845. Epub 2014 Jan 30.
Wnt signaling has been implicated in promoting somatic cell reprogramming. However, its molecular mechanisms remain unknown. Here we report that Wnt/β-catenin enhances iPSCs induction at the early stage of reprogramming. The augmented reprogramming induced by β-catenin is not due to increased total cell population or activation of c-Myc. In addition, β-catenin interacts with reprogramming factors Klf4, Oct4, and Sox2, further enhancing expression of pluripotency circuitry genes. These studies reveal novel mechanisms underlying the regulation of reprogramming somatic cells to pluripotency by Wnt/β-catenin signaling.
Wnt 信号通路被认为在促进体细胞重编程中发挥作用。然而，其具体的分子机制仍不清楚。在这里，我们报道 Wnt/β-catenin 可在重编程的早期阶段增强 iPSCs 的诱导。β-catenin 增强的重编程并不是由于总细胞群体的增加或 c-Myc 的激活。此外，β-catenin 与重编程因子 Klf4、Oct4 和 Sox2 相互作用，进一步增强多能性基因回路的表达。这些研究揭示了 Wnt/β-catenin 信号通路调控体细胞重编程为多能性的新机制。
