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采用触片制备方法对足月胎盘中人绒毛膜促性腺激素α和β链、胎盘催乳素及妊娠特异性β-糖蛋白进行免疫定位。

Immunolocalization of alpha and beta chains of human chorionic gonadotropin, placental lactogen and pregnancy-specific beta-glycoprotein in term placenta by a touch preparation method.

作者信息

Morrish D W, Manickavel V, Jewell L D, Siy O

机构信息

Department of Medicine, University of Alberta, Edmonton, Canada.

出版信息

Histochemistry. 1987;88(1):57-60. doi: 10.1007/BF00490168.

DOI:10.1007/BF00490168
PMID:2449404
Abstract

Touch preparations of human placenta yield cells retaining antigenic reactivity to immunoperoxidase stains for alpha and beta chains of human chorionic gonadotropin, placental lactogen, and pregnancy-specific beta glycoprotein. This method is a rapid and simple alternative to conventional frozen and paraffin-embedded sections for detection of placental peptides.

摘要

人胎盘的触片制备可产生对人绒毛膜促性腺激素α和β链、胎盘催乳素及妊娠特异性β-糖蛋白免疫过氧化物酶染色具有抗原反应性的细胞。该方法是检测胎盘肽的一种快速简便的替代传统冰冻切片和石蜡包埋切片的方法。

相似文献

1
Immunolocalization of alpha and beta chains of human chorionic gonadotropin, placental lactogen and pregnancy-specific beta-glycoprotein in term placenta by a touch preparation method.采用触片制备方法对足月胎盘中人绒毛膜促性腺激素α和β链、胎盘催乳素及妊娠特异性β-糖蛋白进行免疫定位。
Histochemistry. 1987;88(1):57-60. doi: 10.1007/BF00490168.
2
Gastric choriocarcinoma; an immunohistochemical study.胃绒毛膜癌;一项免疫组织化学研究。
Pathol Res Pract. 1986 Aug;181(4):390-6. doi: 10.1016/S0344-0338(86)80073-5.
3
Human chorionic gonadotropin and human placental lactogen in extragonadal tumors. An immunoperoxidase study of ten non-germ cell neoplasms.性腺外肿瘤中的人绒毛膜促性腺激素和人胎盘催乳素。对十种非生殖细胞肿瘤的免疫过氧化物酶研究。
Cancer. 1985 Dec 1;56(11):2674-82. doi: 10.1002/1097-0142(19851201)56:11<2674::aid-cncr2820561125>3.0.co;2-p.
4
Location of the protein hormones of the placenta by the immunoperoxidase technique.用免疫过氧化物酶技术定位胎盘蛋白激素。
Br J Obstet Gynaecol. 1976 Nov;83(11):876-8. doi: 10.1111/j.1471-0528.1976.tb00765.x.
5
Placental proteins (human chorionic gonadotropin, human placental lactogen, pregnancy-specific beta 1-glycoprotein, and placental protein 5) in seminal plasma of normal men and patients with infertility.正常男性和不育症患者精液中的胎盘蛋白(人绒毛膜促性腺激素、人胎盘催乳素、妊娠特异性β1-糖蛋白和胎盘蛋白5)。
Fertil Steril. 1983 May;39(5):704-6. doi: 10.1016/s0015-0282(16)47070-6.
6
[Placental tissue concentration of pregnancy-associated proteins in early and term pregnancies].[早孕期和足月孕期胎盘组织中妊娠相关蛋白的浓度]
Nihon Naibunpi Gakkai Zasshi. 1983 Jul 20;59(7):993-1003. doi: 10.1507/endocrine1927.59.7_993.
7
[Clinical significance of pregnancy-specific beta glycoprotein, human placental lactogen and beta-human chorionic gonadotropin in the serum of patients with trophoblastic diseases].[妊娠特异性β-糖蛋白、人胎盘催乳素及β-人绒毛膜促性腺激素在滋养细胞疾病患者血清中的临床意义]
Zhonghua Yi Xue Za Zhi. 1989 Apr;69(4):206-8, 16.
8
[Pregnancy-associated proteins].
Ann Biol Clin (Paris). 1982;40(4):303-14.
9
Intermediate trophoblast: a distinctive form of trophoblast with specific morphological, biochemical and functional features.中间型滋养细胞:一种具有特定形态、生化和功能特征的独特滋养细胞形式。
Placenta. 1984 Jul-Aug;5(4):349-69. doi: 10.1016/s0143-4004(84)80015-6.
10
Immunofluorescent localization of placental lactogen, chorionic gonadotrophin and its alpha and beta subunits in organ cultures of human placenta.人胎盘器官培养物中胎盘催乳素、绒毛膜促性腺激素及其α和β亚基的免疫荧光定位
Placenta. 1980 Apr-Jun;1(2):135-44. doi: 10.1016/s0143-4004(80)80022-1.

本文引用的文献

1
Immunohistochemical localization of human chorionic gonadotropin.人绒毛膜促性腺激素的免疫组织化学定位
J Exp Med. 1962 Feb 1;115(2):289-94. doi: 10.1084/jem.115.2.289.
2
Predominance of gonadotropin alpha-subunit resulting from preferential loss of hCG beta production in an established cell line.
In Vitro. 1980 Jul;16(7):585-90. doi: 10.1007/BF02618383.
3
Immunoperoxidase localisation of human placental lactogen: a marker for the placental origin of the giant cells in 'syncytial endometritis' of pregnancy.人胎盘催乳素的免疫过氧化物酶定位:妊娠“合体细胞性子宫内膜炎”中巨细胞胎盘起源的标志物。
J Clin Pathol. 1981 Mar;34(3):303-7. doi: 10.1136/jcp.34.3.303.
4
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.注入的辣根过氧化物酶在小鼠肾近端小管吸收的早期阶段:一种新技术的超微结构细胞化学研究
J Histochem Cytochem. 1966 Apr;14(4):291-302. doi: 10.1177/14.4.291.
5
Immunofluorescent localization of chorionic gonadotrophin in monolayer cultures of human trophoblast cells.人滋养层细胞单层培养物中绒毛膜促性腺激素的免疫荧光定位
Nature. 1970 Nov 7;228(5271):561-2. doi: 10.1038/228561a0.
6
Immunofluorescent localisation of a growth hormone-like factor in normal and abnormal syncytiotrophoblast.正常及异常合体滋养层中一种生长激素样因子的免疫荧光定位
J Pathol Bacteriol. 1966 Oct;92(2):395-9. doi: 10.1002/path.1700920217.
7
Localization of human chorionic gonadotropic and somatomammotropic hormones by the peroxidase immunohistoenzymologic method in villi and amniotic epithelium of human placentas (from six weeks to term).采用过氧化物酶免疫组织化学方法对人胎盘绒毛和羊膜上皮中人类绒毛膜促性腺激素和生长催乳素进行定位研究(孕期六周至足月)。
Am J Obstet Gynecol. 1973 Aug 15;116(8):1124-32. doi: 10.1016/0002-9378(73)90948-4.
8
Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae.人足月胎盘细胞滋养层细胞的纯化、特性鉴定及体外分化
Endocrinology. 1986 Apr;118(4):1567-82. doi: 10.1210/endo-118-4-1567.
9
Demonstration of specific secretory granules for human chorionic gonadotropin in placenta.
J Histochem Cytochem. 1987 Jan;35(1):93-101. doi: 10.1177/35.1.2432115.
10
The distribution of placental lactogen in the human term placenta.人足月胎盘催乳素在胎盘内的分布。
Br J Obstet Gynaecol. 1975 Oct;82(10):790-3. doi: 10.1111/j.1471-0528.1975.tb00575.x.