接受辅助生殖治疗的正常体重、超重及肥胖女性卵泡液的脂肪酸组成：一项描述性横断面研究
Fatty acid composition of the follicular fluid of normal weight, overweight and obese women undergoing assisted reproductive treatment: a descriptive cross-sectional study.
作者信息
Valckx Sara D M, Arias-Alvarez Maria, De Pauw Ingrid, Fievez Veerle, Vlaeminck Bruno, Fransen Erik, Bols Peter E J, Leroy Jo L M R
机构信息
Veterinary Physiology and Biochemistry, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.
出版信息
Reprod Biol Endocrinol. 2014 Feb 5;12:13. doi: 10.1186/1477-7827-12-13.
BACKGROUND
It has been well documented that the maturing oocyte is very vulnerable to changes in its micro-environment, the follicular fluid (FF). Recent research has focused on different components within this FF, like hormones, growth factors and metabolites, and how their concentrations are altered by diet and the metabolic health of the mother. It has been proposed that fatty acids (FAs) are potential factors that influence oocyte maturation and subsequent embryo development. However, a thorough study of the specific FF FA composition per lipid fraction and how this may be affected by BMI is currently lacking. Therefore, we investigated the BMI-related concentration of FAs in the phospholipid (PL), cholesteryl-ester (CHE), triglyceride (TG) and non-esterified (NE) lipid fraction in the FF of women undergoing assisted reproductive treatment (ART).
METHODS
In this descriptive cross-sectional study, the FF of normal weight (18.5 ≤ BMI < 25.0 kg/m(2), n = 10), overweight (25.0 ≤ BMI < 30.0 kg/m(2), n = 10) and obese (BMI ≥ 30.0 kg/m(2), n = 10) women, undergoing ART, was sampled and analyzed for 23 specific FAs in the PL, CHE, TG and NEFA fraction, using a gas chromatographic analysis method. Differences between BMI-groups were studied by means of univariate general linear models and post hoc Sheffé tests.
RESULTS
Total FA concentrations in the PL and CHE fraction did not differ between BMI groups. Total TG concentrations tended to differ and total NEFA concentrations differed significantly between BMI groups. Interestingly, 42% and 34% of the total FAs was esterified in the PL and CHE fraction, respectively, while only 10% were present in both the TG and NEFA fraction. Only few individual FA concentrations differed in the PL, CHE and TG fraction between BMI groups, whereas abundant BMI-related differences were found in the NEFA fraction.
CONCLUSIONS
Our data show that differences in BMI are associated with alterations in the FA composition of the FF, an effect most pronounced in the NEFA fraction. These BMI-related variations could possibly affect granulosa cell viability, oocyte developmental competence and subsequent embryo quality possibly explaining differences in oocyte quality in obese patients described by others.
背景
已有充分文献证明，成熟的卵母细胞对其微环境——卵泡液（FF）的变化非常敏感。最近的研究集中在卵泡液中的不同成分，如激素、生长因子和代谢物，以及母亲的饮食和代谢健康如何改变它们的浓度。有人提出脂肪酸（FAs）是影响卵母细胞成熟和随后胚胎发育的潜在因素。然而，目前缺乏对每个脂质组分中卵泡液特定脂肪酸组成的全面研究，以及其如何受体重指数（BMI）影响的研究。因此，我们调查了接受辅助生殖治疗（ART）的女性卵泡液中磷脂（PL）、胆固醇酯（CHE）、甘油三酯（TG）和非酯化（NE）脂质组分中与BMI相关的脂肪酸浓度。
方法
在这项描述性横断面研究中，对接受ART的体重正常（18.5≤BMI＜25.0kg/m²，n = 10）、超重（25.0≤BMI＜30.0kg/m²，n = 10）和肥胖（BMI≥30.0kg/m²，n = 10）女性的卵泡液进行采样，并使用气相色谱分析方法分析PL、CHE、TG和NEFA组分中的23种特定脂肪酸。通过单变量一般线性模型和事后谢费检验研究BMI组之间的差异。
结果
BMI组之间PL和CHE组分中的总脂肪酸浓度没有差异。总TG浓度有差异趋势，总NEFA浓度在BMI组之间有显著差异。有趣的是，分别有42%和34%的总脂肪酸在PL和CHE组分中酯化，而只有10%同时存在于TG和NEFA组分中。BMI组之间PL、CHE和TG组分中只有少数个体脂肪酸浓度存在差异，而在NEFA组分中发现了大量与BMI相关的差异。
结论
我们的数据表明，BMI的差异与卵泡液脂肪酸组成的改变有关，这种影响在NEFA组分中最为明显。这些与BMI相关的变化可能会影响颗粒细胞活力、卵母细胞发育能力和随后的胚胎质量，这可能解释了其他人所描述的肥胖患者卵母细胞质量差异。
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