Kim Hyung Joo, Lee Ji Hyun, Park Hyoung Joon, Cho So-Hyun, Cho Sooyeul, Kim Woo Seong
a Advanced Analysis Team, Toxicological Evaluation and Research Department , National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety , Cheongwon-gun , Chungcheongbuk-do , Korea.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2014;31(5):777-83. doi: 10.1080/19440049.2014.888497. Epub 2014 Mar 26.
Because of the rapid growth in dietary supplement availability and public concern for weight control, the investigation of foods and various dietary supplements illegally adulterated with weight loss compounds has become increasingly important. A total of 29 weight loss compounds, including sennoside, sibutramine, ephedrine and their analogues, found to be adulterated in foods and dietary supplements were simultaneously examined by LC-MS/MS. The 188 samples were collected between 2009 and 2012 in South Korea, and method validation was performed to determine the adulterants to the weight loss compounds. LODs, LOQs and linearity ranged from 0.03 to 7.5 ng ml⁻¹, from 0.08 to 30.00 ng ml⁻¹, and from 0.990 to 0.999, respectively. The results showed that nine weight loss compounds, namely bisacodyl, desmethylsibutramine, didesmethylsibutramine, ephedrine, fluoxetine, pseudoephedrine, sennoside A, sennoside B and sibutramine, were detected in 62 of all collected samples and were found in order of frequency as follows: sibutramine, 25.7%; sennoside A, 22.9%; sennoside B, 20.0%; fluoxetine, 8.6%; desmethylsibutramine, 7.1%; bisacodyl, ephedrine, and pseudoephedrine, 4.3%; and didesmethylsibutramine, 2.9%. Sibutramine, which was the most frequently found adulterant, ranged in levels from 0.03 to 132.40 mg g⁻¹ (2010), from 0.88 to 76.2 mg g⁻¹ (2011), and from 0.07 to 0.24 mg g⁻¹ (2012). Although the concentrations of most compounds ranged widely, some compounds such as bisacodyl and fluoxetine were found at high concentrations in several samples.
由于膳食补充剂的可获得性迅速增长以及公众对体重控制的关注,对非法掺有减肥化合物的食品和各种膳食补充剂的调查变得越来越重要。通过液相色谱-串联质谱法(LC-MS/MS)同时检测了在食品和膳食补充剂中发现的总共29种减肥化合物,包括番泻苷、西布曲明、麻黄碱及其类似物。2009年至2012年期间在韩国收集了188个样品,并进行了方法验证以确定减肥化合物中的掺假物。检测限(LODs)、定量限(LOQs)和线性范围分别为0.03至7.5 ng ml⁻¹、0.08至30.00 ng ml⁻¹和0.990至0.999。结果表明,在所收集的所有样品中的62个中检测到了9种减肥化合物,即比沙可啶、去甲基西布曲明、双去甲基西布曲明、麻黄碱、氟西汀、伪麻黄碱、番泻苷A、番泻苷B和西布曲明,按出现频率依次如下:西布曲明,25.7%;番泻苷A,22.9%;番泻苷B,20.0%;氟西汀,8.6%;去甲基西布曲明,7.1%;比沙可啶、麻黄碱和伪麻黄碱,4.3%;双去甲基西布曲明,2.9%。最常发现的掺假物西布曲明,其含量范围在2010年为0.03至132.40 mg g⁻¹,2011年为0.88至76.2 mg g⁻¹,2012年为0.07至0.24 mg g⁻¹。尽管大多数化合物的浓度范围很广,但在几个样品中发现一些化合物如比沙可啶和氟西汀的浓度很高。