Department of Veterinary Biochemistry, BK21 and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
BMB Rep. 2014 Mar;47(3):141-8. doi: 10.5483/bmbrep.2014.47.3.270.
Bone is an active tissue, in which bone formation by osteoblast is followed by bone resorption by osteoclasts, in a repeating cycle. Proteomics approaches may allow the detection of changes in cell signal transduction, and the regulatory mechanism of cell differentiation. LC-MS/MS-based quantitative methods can be used with labeling strategies, such as SILAC, iTRAQ, TMT and enzymatic labeling. When used in combination with specific protein enrichment strategies, quantitative proteomics methods can identify various signaling molecules and modulators, and their interacting proteins in bone metabolism, to elucidate biological functions for the newly identified proteins in the cellular context. In this article, we will briefly review recent major advances in the application of proteomics for bone biology, especially from the aspect of cellular signaling.
骨骼是一种活跃的组织，成骨细胞通过成骨作用形成骨骼，然后破骨细胞通过破骨作用吸收骨骼，这是一个重复的循环。蛋白质组学方法可以检测细胞信号转导的变化，以及细胞分化的调控机制。基于 LC-MS/MS 的定量方法可以与标记策略（如 SILAC、iTRAQ、TMT 和酶标记）结合使用。当与特定的蛋白质富集策略结合使用时，定量蛋白质组学方法可以鉴定各种信号分子和调节剂及其在骨代谢中的相互作用蛋白，以阐明细胞环境中新鉴定蛋白质的生物学功能。本文将简要综述蛋白质组学在骨生物学中的应用，特别是从细胞信号方面的最新主要进展。
