Ito M, Tazawa T, Shimizu N, Saito A, Sato Y, Nonaka I
Department of Dermatology, Niigata University School of Medicine, Japan.
J Am Acad Dermatol. 1988 Jan;18(1 Pt 1):123-32. doi: 10.1016/s0190-9622(88)70018-3.
An intraepidermally developed epithelial cell tumor, forming multiple nests, was examined to identify its cytologic characteristics. Histochemically, the tumor cells contained neither glycogen nor lipid substance. By N-(7-dimethylamino-3-methyl-4-coumarinyl)maleimide staining, the cytoplasm of the tumor cells in the periphery of each nest was rich in SH groups but not in SS linkages, whereas centrally located homogeneous tumor cells contained SS diffusely but no SH. The tumor cells showed no activity of phosphorylase and a weak activity of succinic dehydrogenase. Immunohistochemically, antihair keratin monoclonal antibodies specific for hair cells decorated the tumor cells, but carcinoembryonic antigen staining showed no positivity. Ultrastructurally, the tumor cells underwent a keratinization forming a fingerprint pattern of keratin filaments; however, membrane-coating granules and marginal bands were not formed. These intraepidermal tumor cells may have cytologic natures similar to those of hair cortical cells. The term intraepidermal pilar epithelioma is proposed as a diagnosis for this tumor.
对一个在表皮内发展的上皮细胞瘤进行了检查，该肿瘤形成多个巢状结构，以确定其细胞学特征。组织化学检查显示，肿瘤细胞既不含糖原也不含脂质物质。通过N-（7-二甲基氨基-3-甲基-4-香豆素基）马来酰亚胺染色，每个巢状结构周边的肿瘤细胞胞质富含巯基（SH）但不含二硫键（SS），而位于中央的均匀肿瘤细胞则弥漫性含有二硫键但不含巯基。肿瘤细胞显示磷酸化酶无活性，琥珀酸脱氢酶活性较弱。免疫组织化学检查中，针对毛细胞的抗毛发角蛋白单克隆抗体可标记肿瘤细胞，但癌胚抗原染色呈阴性。超微结构检查显示，肿瘤细胞发生角化，形成角蛋白丝的指纹状模式；然而，未形成膜包颗粒和边缘带。这些表皮内肿瘤细胞的细胞学性质可能与毛皮质细胞相似。建议将表皮内毛上皮瘤这一术语用于该肿瘤的诊断。
