Peña-Montenegro Tito David, Dussán Jenny
Centro de Investigaciones Microbiológicas - CIMIC, Universidad de los Andes, Bogotá, Colombia .
Stand Genomic Sci. 2013 Oct 2;9(1):42-56. doi: 10.4056/sigs.4227894. eCollection 2013 Oct 16.
Lysinibacillus sphaericus strain OT4b.31 is a native Colombian strain having no larvicidal activity against Culex quinquefasciatus and is widely applied in the bioremediation of heavy-metal polluted environments. Strain OT4b.31 was placed between DNA homology groups III and IV. By gap-filling and alignment steps, we propose a 4,096,672 bp chromosomal scaffold. The whole genome (consisting of 4,856,302 bp long, 94 contigs and 4,846 predicted protein-coding sequences) revealed differences in comparison to the L. sphaericus C3-41 genome, such as syntenial relationships, prophages and putative mosquitocidal toxins. Sphaericolysin B354, the coleopteran toxin Sip1A and heavy metal resistance clusters from nik, ars, czc, cop, chr, czr and cad operons were identified. Lysinibacillus sphaericus OT4b.31 has applications not only in bioremediation efforts, but also in the biological control of agricultural pests.
球形赖氨酸芽孢杆菌菌株OT4b.31是一种哥伦比亚本土菌株，对致倦库蚊没有杀幼虫活性，广泛应用于重金属污染环境的生物修复。菌株OT4b.31位于DNA同源组III和IV之间。通过填补缺口和比对步骤，我们提出了一个4,096,672 bp的染色体支架。整个基因组（由4,856,302 bp长、94个重叠群和4,846个预测的蛋白质编码序列组成）与球形赖氨酸芽孢杆菌C3-41基因组相比显示出差异，如共线性关系、前噬菌体和假定的杀蚊毒素。鉴定出了球形溶菌素B354、鞘翅目毒素Sip1A以及来自nik、ars、czc、cop、chr、czr和cad操纵子的重金属抗性簇。球形赖氨酸芽孢杆菌OT4b.31不仅在生物修复工作中有应用，而且在农业害虫的生物防治中也有应用。
