Mayer B J, Hamaguchi M, Hanafusa H
Rockefeller University, New York, New York 10021.
Nature. 1988 Mar 17;332(6161):272-5. doi: 10.1038/332272a0.
Numerous oncogenes have been isolated from acutely transforming retroviruses. To date, the products of these viral oncogenes have been protein kinases, nuclear proteins, growth factors, or GTP-binding proteins. We have cloned the previously uncharacterized avian sarcoma virus CT10 and sequenced its genome. This virus encodes a protein, p47gag-crk, that has blocks of sequence similarity to the amino-terminal, non-catalytic region of the non-receptor class of tyrosine kinases. In addition, the structure of p47gag-crk has striking similarity to a 180-amino acid region of bovine brain phospholipase C. Biochemical data suggest that p47gag-crk activates one or several endogenous tyrosine kinases.
许多致癌基因已从急性转化逆转录病毒中分离出来。迄今为止，这些病毒致癌基因的产物包括蛋白激酶、核蛋白、生长因子或GTP结合蛋白。我们克隆了之前未被鉴定的禽肉瘤病毒CT10并对其基因组进行了测序。这种病毒编码一种名为p47gag-crk的蛋白质，它与非受体类酪氨酸激酶的氨基末端非催化区域具有序列相似性。此外，p47gag-crk的结构与牛脑磷脂酶C的一个180个氨基酸的区域有显著相似性。生化数据表明，p47gag-crk激活一种或几种内源性酪氨酸激酶。
