Shushimita Shushimita, de Bruijn Marjolein J W, de Bruin Ron W F, IJzermans Jan N M, Hendriks Rudi W, Dor Frank J M F
Department of Surgery, Division of Transplant Surgery, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.
Department of Pulmonary Medicine, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.
PLoS One. 2014 Feb 4;9(2):e87772. doi: 10.1371/journal.pone.0087772. eCollection 2014.
Dietary restriction (DR) delays ageing and extends life span. Both long- and short-term DR, as well as short-term fasting provide robust protection against many "neuronal and surgery related damaging phenomena" such as Parkinson's disease and ischemia-reperfusion injury. The exact mechanism behind this phenomenon has not yet been elucidated. Its anti-inflammatory actions prompted us to thoroughly investigate the consequences of DR and fasting on B and T cell compartments in primary and secondary lymphoid organs of male C57Bl/6 mice. In BM we found that DR and fasting cause a decrease in the total B cell population and arrest early B cell development, while increasing the number of recirculating mature B cells. In the fasting group, a significant reduction in peripheral B cell counts was observed in both spleen and mesenteric lymph nodes (mLN). Thymopoiesis was arrested significantly at double negative DN2 stage due to fasting, whereas DR resulted in a partial arrest of thymocyte development at the DN4 stage. Mature CD3(+) T cell populations were increased in BM and decreased in both spleen and mLN. Thus, DR arrests B cell development in the BM but increases the number of recirculating mature B cells. DR also arrests maturation of T cells in thymus, resulting in depletion of mature T cells from spleen and mLN while recruiting them to the BM. The functional relevance in relation to protection against organ damage needs to be determined.
饮食限制（DR）可延缓衰老并延长寿命。长期和短期的饮食限制以及短期禁食，都能为抵御许多“与神经元和手术相关的损伤现象”（如帕金森病和缺血再灌注损伤）提供强大的保护作用。这一现象背后的确切机制尚未阐明。其抗炎作用促使我们深入研究饮食限制和禁食对雄性C57Bl/6小鼠初级和次级淋巴器官中B细胞和T细胞区室的影响。在骨髓中，我们发现饮食限制和禁食会导致B细胞总数减少，并使早期B细胞发育停滞，同时循环成熟B细胞数量增加。在禁食组中，脾脏和肠系膜淋巴结（mLN）中的外周B细胞计数均显著降低。由于禁食，胸腺细胞生成在双阴性DN2阶段显著停滞，而饮食限制则导致胸腺细胞发育在DN4阶段部分停滞。骨髓中成熟CD3(+) T细胞群体增加，而脾脏和mLN中的成熟CD3(+) T细胞群体减少。因此，饮食限制会使骨髓中的B细胞发育停滞，但会增加循环成熟B细胞的数量。饮食限制还会使胸腺中T细胞的成熟停滞，导致脾脏和mLN中成熟T细胞减少，同时将它们招募到骨髓中。其与抵御器官损伤相关的功能相关性尚待确定。
