Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, Jiangsu, China; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi 214081, China.
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi 214081, China.
Ecotoxicol Environ Saf. 2014 Mar;101:1-6. doi: 10.1016/j.ecoenv.2013.10.020. Epub 2014 Jan 7.
The chronic effect of methomyl on the antioxidant system in tilapia (Oreochromis niloticus) was investigated. Fish were exposed to sub-lethal concentrations of 0.2, 2, 20 and 200μgL(-1) for 30 days, and then transferred to methomyl-free water for 18 days. Hepatic antioxidant parameters, including Glutathione-S-transferase (GST), Glutathione peroxidase (GPx), Glutathione reductase (GR), Reduced glutathione (GSH) and oxidized glutathione (GSSG), were measured at 10min (day 0), 6, 12, 18, 24 and 30 days after starting the experiment and at 18 days after transferring to methomyl-free water. There were no significant changes in enzymatic activity and content of antioxidants in liver of tilapia exposed to 0.2μgL(-1) methomyl compared to controls. However, the results showed significant increases in activities of GST, GR, GPx and levels of GSSG accompanied by a decrease in GSH levels following methomyl exposure in tilapia to 2, 20 or 200μgL(-1) over the 30-day exposure period and the highest induction rates in GST, GR, GPx and GSSG were 150.87%, 163.21%, 189.76%, and 179.56% of the control respectively, and the highest inhibition rate in GSH was 50.67% of the control, suggesting the presence of oxidative stress. Thus it would appear that the 0.2μgL(-1) methomyl might be considered as the no observed adverse effect level (NOAEL). Recovery data showed that the effects produced by lower concentration of methomyl 20μgL(-1) were reversible but not at the higher 200μgL(-1) concentration.
研究了灭多威对罗非鱼（Oreochromis niloticus）抗氧化系统的慢性影响。鱼暴露于亚致死浓度 0.2、2、20 和 200μg/L 下 30 天，然后转移到无灭多威的水中 18 天。在实验开始后 10min（第 0 天）、6、12、18、24 和 30 天以及转移到无灭多威的水中 18 天后，测量了肝脏中的抗氧化参数，包括谷胱甘肽-S-转移酶（GST）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）、还原型谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）。与对照组相比，暴露于 0.2μg/L 灭多威的罗非鱼肝脏中抗氧化酶的活性和含量没有显著变化。然而，结果表明，随着暴露时间的延长，2、20 或 200μg/L 灭多威暴露组罗非鱼 GST、GR、GPx 的活性和 GSSG 的水平显著升高，GSH 的水平显著降低，在 GST、GR、GPx 和 GSSG 中的诱导率最高分别为对照组的 150.87%、163.21%、189.76%和 179.56%，GSH 的最高抑制率为对照组的 50.67%，表明存在氧化应激。因此，0.2μg/L 灭多威可能被认为是无观察不良效应水平（NOAEL）。恢复数据表明，较低浓度（20μg/L）灭多威产生的影响是可逆的，但在较高浓度（200μg/L）下则不可逆转。
